Anti-M Cadherin antibody (ab217861)
Key features and details
- Rabbit polyclonal to M Cadherin
- Suitable for: IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-M Cadherin antibody
See all M Cadherin primary antibodies -
Description
Rabbit polyclonal to M Cadherin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Human -
Immunogen
Synthetic peptide within Human M Cadherin aa 350-450 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P55291 -
Positive control
- Rat cardiac muscle tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X
Aqueous buffered solution -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab217861 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100 - 1/500.
When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
Notes |
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IHC-P
1/100 - 1/500. When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
Target
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Function
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation. -
Tissue specificity
Expressed in the brain and cerebellum. -
Involvement in disease
A chromosomal aberration involving CDH15 and KIRREL3 is found in a patient with severe mental retardation and dysmorphic facial features. Translocation t(11;16)(q24.2;q24).
Mental retardation, autosomal dominant 3 -
Sequence similarities
Contains 5 cadherin domains. -
Domain
Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1013 Human
- Entrez Gene: 12555 Mouse
- Entrez Gene: 361432 Rat
- Omim: 114019 Human
- SwissProt: P55291 Human
- SwissProt: P33146 Mouse
- Unigene: 148090 Human
- Unigene: 1976 Mouse
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Alternative names
- CAD15_HUMAN antibody
- Cadherin 14 antibody
- Cadherin 15 antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat cardiac muscle tissue labeling M Cadherin with ab217861 at 1/200 dilution, followed Goat Anti-Rabbit IgG, Cy3 conjugated secondary antibody at 1/200 dilution and DAPI staining.
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat cardiac muscle tissue labeling M Cadherin with ab217861 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab217861 has not yet been referenced specifically in any publications.