Anti-M Cadherin antibody [EP5756] (ab129078)
Key features and details
- Rabbit monoclonal [EP5756] to M Cadherin
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-M Cadherin antibody [EP5756]
See all M Cadherin primary antibodies -
Description
Rabbit monoclonal [EP5756] to M Cadherin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human M Cadherin aa 100-200. The exact sequence is proprietary.
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Positive control
- WB: A673, Human cerebellum, Fetal heart, Fetal brain and 293T cell lysates; Mouse brain, heart, kidney and spleen tissues; Rat brain, kidney and spleen tissues.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP5756 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 89 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 89 kDa). |
Target
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Function
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation. -
Tissue specificity
Expressed in the brain and cerebellum. -
Involvement in disease
A chromosomal aberration involving CDH15 and KIRREL3 is found in a patient with severe mental retardation and dysmorphic facial features. Translocation t(11;16)(q24.2;q24).
Mental retardation, autosomal dominant 3 -
Sequence similarities
Contains 5 cadherin domains. -
Domain
Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1013 Human
- Entrez Gene: 12555 Mouse
- Entrez Gene: 361432 Rat
- Omim: 114019 Human
- SwissProt: P55291 Human
- SwissProt: P33146 Mouse
- Unigene: 148090 Human
- Unigene: 1976 Mouse
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Alternative names
- CAD15_HUMAN antibody
- Cadherin 14 antibody
- Cadherin 15 antibody
see all
Images
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All lanes : Anti-M Cadherin antibody [EP5756] (ab129078) at 0.089 µg/ml
Lane 1 : Rat Brain Tissue
Lane 2 : Rat Heart Tissue
Lane 3 : Rat Kidney Tissue
Lane 4 : Rat Spleen Tissue
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 89 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer and concentration: 5% NFDM /TBST
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All lanes : Anti-M Cadherin antibody [EP5756] (ab129078) at 0.089 µg/ml
Lane 1 : Mouse Brain Tissue
Lane 2 : Mouse Heart Tissue
Lane 3 : Mouse Kidney Tissue
Lane 4 : Mouse Spleen Tissue
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 89 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer and concentration: 5% NFDM /TBST
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All lanes : Anti-M Cadherin antibody [EP5756] (ab129078) at 1/1000 dilution
Lane 1 : A673 cell lysate
Lane 2 : Human cerebellum cell lysate
Lane 3 : Human fetal heart cell lysate
Lane 4 : Human fetal brain cell lysate
Lane 5 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-rabbit HRP
Predicted band size: 89 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab129078 has not yet been referenced specifically in any publications.