Anti-M Cadherin antibody [EP5756] (ab129078)
![Western blot - Anti-M Cadherin antibody [EP5756] (ab129078)](https://www.abcam.com/ps/products/129/ab129078/Images/ab129078-383901-WB2anti-m-cadherin-antibody-ep5756-western-blot-rat.jpg "Western blot - Anti-M Cadherin antibody [EP5756] (ab129078)")
Key features and details
- Rabbit monoclonal [EP5756] to M Cadherin
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-M Cadherin antibody [EP5756]
See all M Cadherin primary antibodies -
Description
Rabbit monoclonal [EP5756] to M Cadherin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human M Cadherin aa 100-200. The exact sequence is proprietary.
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Positive control
- WB: A673, Human cerebellum, Fetal heart, Fetal brain and 293T cell lysates; Mouse brain, heart, kidney and spleen tissues; Rat brain, kidney and spleen tissues.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP5756 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab129078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 89 kDa). |
Target
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Function
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation. -
Tissue specificity
Expressed in the brain and cerebellum. -
Involvement in disease
A chromosomal aberration involving CDH15 and KIRREL3 is found in a patient with severe mental retardation and dysmorphic facial features. Translocation t(11;16)(q24.2;q24).
Mental retardation, autosomal dominant 3 -
Sequence similarities
Contains 5 cadherin domains. -
Domain
Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1013 Human
- Entrez Gene: 12555 Mouse
- Entrez Gene: 361432 Rat
- Omim: 114019 Human
- SwissProt: P55291 Human
- SwissProt: P33146 Mouse
- Unigene: 148090 Human
- Unigene: 1976 Mouse
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Alternative names
- CAD15_HUMAN antibody
- Cadherin 14 antibody
- Cadherin 15 antibody
see all
Images
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Western blot - Anti-M Cadherin antibody [EP5756] (ab129078)All lanes : Anti-M Cadherin antibody [EP5756] (ab129078) at 0.089 µg/ml
Lane 1 : Rat Brain Tissue
Lane 2 : Rat Heart Tissue
Lane 3 : Rat Kidney Tissue
Lane 4 : Rat Spleen Tissue
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 89 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer and concentration: 5% NFDM /TBST
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![Western blot - Anti-M Cadherin antibody [EP5756] (ab129078)](https://www.abcam.com/ps/products/129/ab129078/Images/ab129078-383900-WB1anti-m-cadherin-antibody-ep5756-western-blot-mouse.jpg)
Western blot - Anti-M Cadherin antibody [EP5756] (ab129078)All lanes : Anti-M Cadherin antibody [EP5756] (ab129078) at 0.089 µg/ml
Lane 1 : Mouse Brain Tissue
Lane 2 : Mouse Heart Tissue
Lane 3 : Mouse Kidney Tissue
Lane 4 : Mouse Spleen Tissue
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 89 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer and concentration: 5% NFDM /TBST
-
![Western blot - Anti-M Cadherin antibody [EP5756] (ab129078)](https://www.abcam.com/ps/products/129/ab129078/Images/ab129078-143028-anti-m-cadherin-antibody-ep5756-western-blot.jpg)
Western blot - Anti-M Cadherin antibody [EP5756] (ab129078)All lanes : Anti-M Cadherin antibody [EP5756] (ab129078) at 1/1000 dilution
Lane 1 : A673 cell lysate
Lane 2 : Human cerebellum cell lysate
Lane 3 : Human fetal heart cell lysate
Lane 4 : Human fetal brain cell lysate
Lane 5 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-rabbit HRP
Predicted band size: 89 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Datasheets and documents
References (0)
ab129078 has not yet been referenced specifically in any publications.
