Anti-M Cadherin antibody [EP5756] (ab129078)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-M Cadherin antibody [EP5756]
See all M Cadherin primary antibodies -
Description
Rabbit monoclonal [EP5756] to M Cadherin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human M Cadherin aa 100-200. The exact sequence is proprietary.
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Positive control
- A673, Human cerebellum, Fetal heart, Fetal brain and 293T cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP5756 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab129078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 89 kDa). |
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
Target
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Function
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation. -
Tissue specificity
Expressed in the brain and cerebellum. -
Involvement in disease
A chromosomal aberration involving CDH15 and KIRREL3 is found in a patient with severe mental retardation and dysmorphic facial features. Translocation t(11;16)(q24.2;q24).
Mental retardation, autosomal dominant 3 -
Sequence similarities
Contains 5 cadherin domains. -
Domain
Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1013 Human
- Entrez Gene: 12555 Mouse
- Entrez Gene: 361432 Rat
- Omim: 114019 Human
- SwissProt: P55291 Human
- SwissProt: P33146 Mouse
- Unigene: 148090 Human
- Unigene: 1976 Mouse
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Alternative names
- CAD15_HUMAN antibody
- Cadherin 14 antibody
- Cadherin 15 antibody
see all
Images
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![Western blot - Anti-M Cadherin antibody [EP5756] (ab129078)](https://www.abcam.com/ps/products/129/ab129078/Images/ab129078-143028-anti-m-cadherin-antibody-ep5756-western-blot.jpg)
Western blot - Anti-M Cadherin antibody [EP5756] (ab129078)All lanes : Anti-M Cadherin antibody [EP5756] (ab129078) at 1/1000 dilution
Lane 1 : A673 cell lysate
Lane 2 : Human cerebellum cell lysate
Lane 3 : Human fetal heart cell lysate
Lane 4 : Human fetal brain cell lysate
Lane 5 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-rabbit HRP
Predicted band size: 89 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
References
ab129078 has not yet been referenced specifically in any publications.
