Product nameAnti-M Cadherin antibody [EP5756]
See all M Cadherin primary antibodies
DescriptionRabbit monoclonal [EP5756] to M Cadherin
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human M Cadherin aa 100-200. The exact sequence is proprietary.
- A673, Human cerebellum, Fetal heart, Fetal brain and 293T cell lysates.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab129078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 89 kDa).|
FunctionCadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation.
Tissue specificityExpressed in the brain and cerebellum.
Involvement in diseaseA chromosomal aberration involving CDH15 and KIRREL3 is found in a patient with severe mental retardation and dysmorphic facial features. Translocation t(11;16)(q24.2;q24).
Mental retardation, autosomal dominant 3
Sequence similaritiesContains 5 cadherin domains.
DomainThree calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.
Cellular localizationCell membrane.
- Information by UniProt
- CAD15_HUMAN antibody
- Cadherin 14 antibody
- Cadherin 15 antibody
All lanes : Anti-M Cadherin antibody [EP5756] (ab129078) at 1/1000 dilution
Lane 1 : A673 cell lysate
Lane 2 : Human cerebellum cell lysate
Lane 3 : Human fetal heart cell lysate
Lane 4 : Human fetal brain cell lysate
Lane 5 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-rabbit HRP
Predicted band size: 89 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
ab129078 has not yet been referenced specifically in any publications.