Anti-M-CSF antibody (ab99178)
Key features and details
- Rabbit polyclonal to M-CSF
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-M-CSF antibody
See all M-CSF primary antibodies -
Description
Rabbit polyclonal to M-CSF -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Dog, Pig, Macaque monkey, Gorilla -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- This antibody gave a positive signal in the following human tissue lysates: Bone Marrow; Spleen; Thymus; Tonsil. It also gave a positive result in FFPE human tonsil tissue sections.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (2) |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 60 kDa).
|
Notes |
---|
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 60 kDa). |
Target
-
Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. -
Post-translational
modificationsGlycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
-
Database links
- Entrez Gene: 1435 Human
- Entrez Gene: 12977 Mouse
- Omim: 120420 Human
- SwissProt: P09603 Human
- SwissProt: P07141 Mouse
- Unigene: 591402 Human
- Unigene: 795 Mouse
-
Alternative names
- Colony stimulating factor 1 (macrophage) antibody
- Colony stimulating factor 1 antibody
- Colony stimulating factor macrophage specific antibody
see all
Images
-
IHC image of MCSF staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab99178, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Anti-M-CSF antibody (ab99178) at 1 µg/ml + Mouse Spleen lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Predicted band size: 60 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesBlocking buffer: 2% BSA
Gel type: MOPS -
All lanes : Anti-M-CSF antibody (ab99178) at 1 µg/ml
Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lane 3 : Human thymus tissue lysate - total protein (ab30146)
Lane 4 : Tonsil (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Additional bands at: 170 kDa, 52 kDa (possible cleavage fragment), 98 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
MCSF contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
ab99178 staining MCSF in mouse skin wound tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 for 10 minutes and blocked with 1% BSA for 30 minutes at 25°C; antigen retrieval was by heat mediation in a citrate-EDTA buffer. Samples were incubated with primary antibody (1/100 in TBS pH 7.6) for 1 hour at 25°C. A Biotin-conjugated donkey anti-rabbit IgG (H+L) polyclonal (1/300) was used as the secondary antibody.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (8)
ab99178 has been referenced in 8 publications.
- Samain R et al. Pharmacologic Normalization of Pancreatic Cancer-Associated Fibroblast Secretome Impairs Prometastatic Cross-Talk With Macrophages. Cell Mol Gastroenterol Hepatol 11:1405-1436 (2021). PubMed: 33482394
- Sawaied A et al. The Presence of Colony-Stimulating Factor-1 and Its Receptor in Different Cells of the Testis; It Involved in the Development of Spermatogenesis In Vitro. Int J Mol Sci 22:N/A (2021). PubMed: 33652607
- Wada Y et al. Reno-protective effect of IL-34 inhibition on cisplatin-induced nephrotoxicity in mice. PLoS One 16:e0245340 (2021). PubMed: 33428678
- Liu R et al. CircCDC45 promotes the malignant progression of glioblastoma by modulating the miR-485-5p/CSF-1 axis. BMC Cancer 21:1090 (2021). PubMed: 34627193
- Galliverti G et al. Myeloid Cells Orchestrate Systemic Immunosuppression, Impairing the Efficacy of Immunotherapy against HPV+ Cancers. Cancer Immunol Res 8:131-145 (2020). PubMed: 31771984
- Mao Z et al. MicroRNA-155 inhibition up-regulates LEPR to inhibit osteoclast activation and bone resorption via activation of AMPK in alendronate-treated osteoporotic mice. IUBMB Life N/A:N/A (2019). PubMed: 31317664
- Moon HG et al. Airway Epithelial Cell-Derived Colony Stimulating Factor-1 Promotes Allergen Sensitization. Immunity 49:275-287.e5 (2018). PubMed: 30054206
- de Jong RJ et al. Protective Aptitude of Annexin A1 in Arterial Neointima Formation in Atherosclerosis-Prone Mice-Brief Report. Arterioscler Thromb Vasc Biol 37:312-315 (2017). PubMed: 28062503