Key features and details
- Rabbit polyclonal to M-CSF (Biotin)
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
FormLyophilized:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab84262 is purified by affinity chromatography and then biotinylated and is sterile filtered.
sELISA pair antibody
Our Abpromise guarantee covers the use of ab84262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to M-CSF (ab9693). Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human MCSF. Can be used as detection antibody when paired with ab9693.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 60 kDa. The detection limit for recombinant human MCSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
FunctionGranulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
modificationsGlycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.
Cellular localizationCell membrane and Secreted > extracellular space.
- Information by UniProt
- Colony stimulating factor 1 (macrophage) antibody
- Colony stimulating factor 1 antibody
- Colony stimulating factor macrophage specific antibody
ab84262 has not yet been referenced specifically in any publications.