Product nameAnti-M-CSF antibody [EP1179Y]
See all M-CSF primary antibodies
DescriptionRabbit monoclonal [EP1179Y] to M-CSF
SpecificityThis antibody fails to detect endogenous natural samples in WB.
Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Sheep, Cow, Chimpanzee
Synthetic peptide within Human M-CSF aa 50-150. The exact sequence is proprietary.
Database link: P09603
- WB: Recombinant MCSF IHC-P: Human tonsil tissue.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab52864 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 60 kDa.
This antibody fails to detect endogenous natural samples in WB.
For unpurified use at 1/50 dilution.
|IHC-P||1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For unpurified use at 1/50 - 1/100 dilution.
For unpurified use at 1/250 - 1/500 dilution.
FunctionGranulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
modificationsGlycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.
Cellular localizationCell membrane and Secreted > extracellular space.
- Information by UniProt
- Colony stimulating factor 1 (macrophage) antibody
- Colony stimulating factor 1 antibody
- Colony stimulating factor macrophage specific antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling M-CSF with Purified ab52864 at 1:500 dilution (1.52 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)
Immunocytochemistry/ Immunofluorescence analysis of THP-1 (Human monocytic leukemia monocyte) cells labeling M-CSF with Purified ab52864 at 1:100 (7.6 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Anti-M-CSF antibody [EP1179Y] (ab52864) at 1/2000 dilution (Purified) + Human M-CSF (aa 33-190) recombinant protein at 0.015 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 60 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Blocking buffer: 5% NFDM/TBST.
Flow Cytometry analysis of THP-1 (Human monocytic leukemia monocyte) cells labeling M-CSF with purified ab52864 at 1:80 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Formalin-fixed, paraffin-embedded human tonsil tissue stained for M-CSF with unpurified ab52864 (1/50 dilution) in immunohistochemical analysis.
Anti-M-CSF antibody [EP1179Y] (ab52864) at 1/50000 dilution (unpurified) + recombinant MCSF at 0.005 µg
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 60 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
ab52864 (purified) at 1:40 dilution (2µg) immunoprecipitating M-CSF in THP-1 whole cell lysate.
Lane 1 (input): THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10µg
Lane 2 (+): ab52864 & THP-1 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52864 in THP-1 whole cell lysate
For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
This product has been referenced in:
- Baghdadi M et al. High co-expression of IL-34 and M-CSF correlates with tumor progression and poor survival in lung cancers. Sci Rep 8:418 (2018). Read more (PubMed: 29323162) »
- Wang H et al. Interactions between colon cancer cells and tumor-infiltrated macrophages depending on cancer cell-derived colony stimulating factor 1. Oncoimmunology 5:e1122157 (2016). Read more (PubMed: 27141406) »