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Synthetic peptide within Human M-CSF aa 50-150. The exact sequence is proprietary.
Database link: P09603
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab52864 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 60 kDa.
This antibody fails to detect endogenous natural samples in WB.
For unpurified use at 1/50 dilution.
|IHC-P||1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols.
For unpurified use at 1/250 - 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling M-CSF with Purified ab52864 at 1:500 dilution (1.52 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)
Immunocytochemistry/ Immunofluorescence analysis of THP-1 (Human monocytic leukemia monocyte) cells labeling M-CSF with Purified ab52864 at 1:100 (7.6 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry analysis of THP-1 (Human monocytic leukemia monocyte) cells labeling M-CSF with purified ab52864 at 1:80 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Formalin-fixed, paraffin-embedded human tonsil tissue stained for M-CSF with unpurified ab52864 (1/50 dilution) in immunohistochemical analysis.
ab52864 (purified) at 1:40 dilution (2µg) immunoprecipitating M-CSF in THP-1 whole cell lysate.
Lane 1 (input): THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10µg
Lane 2 (+): ab52864 & THP-1 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52864 in THP-1 whole cell lysate
For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"