Recombinant
RabMAb

Recombinant Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (ab232165)

Overview

  • Product name

    Anti-M-CSF antibody [EP1179Y] - BSA and Azide free
    See all M-CSF primary antibodies
  • Description

    Rabbit monoclonal [EP1179Y] to M-CSF - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human M-CSF aa 50-150. The exact sequence is proprietary.
    Database link: P09603

  • Positive control

    • IHC-P: Human tonsil tissue.
  • General notes

    Ab232165 is the carrier-free version of ab52864. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab232165 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 60 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
  • Post-translational
    modifications

    Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
    Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.
  • Cellular localization

    Cell membrane and Secreted > extracellular space.
  • Information by UniProt
  • Database links

  • Alternative names

    • Colony stimulating factor 1 (macrophage) antibody
    • Colony stimulating factor 1 antibody
    • Colony stimulating factor macrophage specific antibody
    • CSF 1 antibody
    • CSF-1 antibody
    • CSF1 antibody
    • CSF1_HUMAN antibody
    • Csfm antibody
    • Lanimostim antibody
    • M CSF antibody
    • M-CSF antibody
    • Macrophage Colony Stimulating Factor 1 antibody
    • Macrophage colony stimulating factor antibody
    • MCSF antibody
    • MGC31930 antibody
    • Processed macrophage colony-stimulating factor 1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling M-CSF with Purified ab52864 at 1:500 dilution (1.52 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52864).

  • ab52864 (purified) at 1:40 dilution (2µg) immunoprecipitating M-CSF in THP-1 whole cell lysate.
    Lane 1 (input): THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10µg
    Lane 2 (+): ab52864 & THP-1 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52864 in THP-1 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52864).

  • Flow Cytometry analysis of THP-1 (Human monocytic leukemia monocyte) cells labeling M-CSF with purified ab52864 at 1:80 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52864).

  • Immunocytochemistry/ Immunofluorescence analysis of THP-1 (Human monocytic leukemia monocyte) cells labeling M-CSF with Purified ab52864 at 1:100 (7.6 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52864).

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for M-CSF with ab52864  (1/50 dilution) in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52864).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

References

ab232165 has not yet been referenced specifically in any publications.

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