Recombinant
RabMAb

Recombinant Anti-M-CSF antibody [EP1179Y] (HRP) (ab206234)

Overview

  • Product name

    Anti-M-CSF antibody [EP1179Y] (HRP)
    See all M-CSF primary antibodies
  • Description

    Rabbit monoclonal [EP1179Y] to M-CSF (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Sheep, Cow, Chimpanzee
  • Immunogen

    Synthetic peptide within Human M-CSF aa 50-150. The exact sequence is proprietary.
    Database link: P09603

  • Positive control

    • WB: M-CSF recombinant protein. IHC-P: FFPE human tonsil (normal) tissue sections.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab206234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.

WB 1/5000. Detects a band of approximately 19 kDa (predicted molecular weight: 60 kDa).

Target

  • Function

    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
  • Post-translational
    modifications

    Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
    Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.
  • Cellular localization

    Cell membrane and Secreted > extracellular space.
  • Information by UniProt
  • Database links

  • Alternative names

    • Colony stimulating factor 1 (macrophage) antibody
    • Colony stimulating factor 1 antibody
    • Colony stimulating factor macrophage specific antibody
    • CSF 1 antibody
    • CSF-1 antibody
    • CSF1 antibody
    • CSF1_HUMAN antibody
    • Csfm antibody
    • Lanimostim antibody
    • M CSF antibody
    • M-CSF antibody
    • Macrophage Colony Stimulating Factor 1 antibody
    • Macrophage colony stimulating factor antibody
    • MCSF antibody
    • MGC31930 antibody
    • Processed macrophage colony-stimulating factor 1 antibody
    see all

Images

  • Anti-M-CSF antibody [EP1179Y] (HRP) (ab206234) at 1/5000 dilution + M-CSF Recombinant Protein at 0.1 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 60 kDa
    Observed band size: 19 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab206234 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • IHC image of MCSF staining in a section of formalin-fixed paraffin-embedded normal human tonsil tissue*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab206234, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References

ab206234 has not yet been referenced specifically in any publications.

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