Overview

  • Product name

    Anti-M-CSF antibody [EPR20948]
    See all M-CSF primary antibodies
  • Description

    Rabbit monoclonal [EPR20948] to M-CSF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human M-CSF aa 200-300. The exact sequence is proprietary.
    Database link: P09603

  • Positive control

    • WB: U937, P388D1, J774A.1, Jurkat, MDA-MB-231, K562, C6, PC-12 and NIH/3T3 whole cell lysates; Human tonsil, brain and kidney lysates. IHC-P: Human colon and bladder cancer tissues; Mouse and rat colon tissues. ICC/IF: Jurkat cells. Flow Cyt: Jurkat cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab233387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 60 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.

Target

  • Function

    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
  • Post-translational
    modifications

    Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
    Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.
  • Cellular localization

    Cell membrane and Secreted > extracellular space.
  • Information by UniProt
  • Database links

  • Alternative names

    • Colony stimulating factor 1 (macrophage) antibody
    • Colony stimulating factor 1 antibody
    • Colony stimulating factor macrophage specific antibody
    • CSF 1 antibody
    • CSF-1 antibody
    • CSF1 antibody
    • CSF1_HUMAN antibody
    • Csfm antibody
    • Lanimostim antibody
    • M CSF antibody
    • M-CSF antibody
    • Macrophage Colony Stimulating Factor 1 antibody
    • Macrophage colony stimulating factor antibody
    • MCSF antibody
    • MGC31930 antibody
    • Processed macrophage colony-stimulating factor 1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human colon tissue labeling M-CSF with ab233387 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin. Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Positive staining on stromal cells (arrows) and weak staining on epithelium of human colon (PMID: 15519852; PMID: 11745698).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • All lanes : Anti-M-CSF antibody [EPR20948] (ab233387) at 1/1000 dilution

    Lane 1 : U937 (human histiocytic lymphoma cell line) whole cell lysate
    Lane 2 : P388D1 (mouse lymphoma monocyte; macrophage cell line) whole cell lysate
    Lanes 3 & 7 : J774A.1 (mouse reticulum cell sarcoma monocyte macrophage cell line) whole cell lysate
    Lane 4 : Human tonsil lysate
    Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 6 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 8 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Predicted band size: 60 kDa
    Observed band size: 43 kDa
    why is the actual band size different from the predicted?



    Exposure time : Lanes 1-4: 3 minutes; Lanes 5: 6 seconds; Lanes 6-8: 26 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 21502940; PMID: 3264877).

  • All lanes : Anti-M-CSF antibody [EPR20948] (ab233387) at 1/1000 dilution

    Lane 1 : C6 (rat glial tumor cell line) whole cell lysate
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 4 : Human brain lysate
    Lane 5 : Human kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
    Lanes 4-5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 60 kDa
    Observed band size: 43 kDa why is the actual band size different from the predicted?



    Exposure time : Lanes 1-3: 3 minutes; Lanes 4-5:10 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 21502940; PMID: 3264877).

  • Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling M-CSF with ab233387 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.  Counter stained with hematoxylin. Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Membranous and weak cytoplasmic staining on human bladder cancer (PMID: 25082815; PMID: 25667468) is observed.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling M-CSF with ab233387 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin. Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Positive staining on stromal cells (arrows) and weak staining on epithelium of mouse colon (PMID: 15519852; PMID: 11745698).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling M-CSF with ab233387 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin. Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Positive staining on stromal cells (arrows) and weak staining on epithelium of rat colon (PMID: 15519852; PMID: 11745698)

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunofluorescent analysis of 100% methanol-fixed Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling M-CSF with ab233387 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in Jurkat cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling M-CSF with ab233387 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

References

ab233387 has not yet been referenced specifically in any publications.

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