Recombinant
RabMAb

Recombinant Anti-M-CSF antibody [EPR20948] - BSA and Azide free (ab234259)

Rabbit recombinant monoclonal M-CSF antibody [EPR20948]. Validated in WB, IHC, Flow Cyt, ICC/IF and tested in Mouse, Rat, Human.

Overview

  • Product name

    Anti-M-CSF antibody [EPR20948] - BSA and Azide free
    See all M-CSF primary antibodies
  • Description

    Rabbit monoclonal [EPR20948] to M-CSF - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human M-CSF aa 200-300. The exact sequence is proprietary.
    Database link: P09603

  • Positive control

    • IHC-P: Human colon tissue.
  • General notes

    Ab234259 is the carrier-free version of ab233387. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab234259 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab234259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 43 kDa (predicted molecular weight: 60 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
  • Post-translational
    modifications

    Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
    Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.
  • Cellular localization

    Cell membrane and Secreted > extracellular space.
  • Information by UniProt
  • Database links

  • Alternative names

    • Colony stimulating factor 1 (macrophage) antibody
    • Colony stimulating factor 1 antibody
    • Colony stimulating factor macrophage specific antibody
    • CSF 1 antibody
    • CSF-1 antibody
    • CSF1 antibody
    • CSF1_HUMAN antibody
    • Csfm antibody
    • Lanimostim antibody
    • M CSF antibody
    • M-CSF antibody
    • Macrophage Colony Stimulating Factor 1 antibody
    • Macrophage colony stimulating factor antibody
    • MCSF antibody
    • MGC31930 antibody
    • Processed macrophage colony-stimulating factor 1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human colon tissue labeling M-CSF with ab233387 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin. Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Positive staining on stromal cells (arrows) and weak staining on epithelium of human colon (PMID: 15519852; PMID: 11745698).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233387).

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling M-CSF with ab233387 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin. Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Positive staining on stromal cells (arrows) and weak staining on epithelium of mouse colon (PMID: 15519852; PMID: 11745698).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233387).

  • Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling M-CSF with ab233387 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin. Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Positive staining on stromal cells (arrows) and weak staining on epithelium of rat colon (PMID: 15519852; PMID: 11745698)

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233387).

  • Immunofluorescent analysis of 100% methanol-fixed Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling M-CSF with ab233387 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in Jurkat cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233387).

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling M-CSF with ab233387 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233387).

  • Immunohistochemical analysis of paraffin-embedded human colon tissue labeling M-CSF with ab233387 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on stromal cells and weak staining on epithelium of human colon (PMID: 15519852; PMID:11745698) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233387).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab234259 has not yet been referenced specifically in any publications.

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