Product nameM-CSF overexpression 293T lysate (whole cell)
See all M-CSF lysates
ab94076 is a 293T cell transfected lysate in which Human MCSF has been transiently over-expressed using a pCMV-MCSF plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailes about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. PTM: Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate. Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.
Our Abpromise guarantee covers the use of ab94076 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94076 at 15μg/lane on an SDS-PAGE gel.
All lanes : Anti-MCSF antibody (ab66236) at 1/500 dilution
Lane 1 :
M-CSF overexpression 293T lysate (whole cell) (ab94076)
Lane 2 : 293T non transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94076 has not yet been referenced specifically in any publications.