Product nameAnti-M-CSF/CSF1 antibody (Biotin)
DescriptionGoat polyclonal to M-CSF/CSF1 (Biotin)
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Mouse
FormLyophilised:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.20
Constituents: PBS, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab271262 in the following tested applications.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 60 kDa.|
|ELISA||Use a concentration of 0.25 - 1 µg/ml.|
RelevanceCytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.
Cellular localizationCell membrane; Single-pass membrane protein
- Colony stimulating factor 1 (macrophage) antibody
- CSF-1 antibody
- CSF1 antibody
Western blot analysis of varying levels of Recombinant murine M-CSF protein using ab271262 at 0.1 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant M-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Mouse M-CSF was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271262. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mM-CSF protein.
ab271262 has not yet been referenced specifically in any publications.