Anti-M13 + Fd Bacteriophage Coat Proteins antibody (ab6188)
- Datasheet
- Specific References (8)
- Protocols
Overview
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Product nameAnti-M13 + Fd Bacteriophage Coat Proteins antibody
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DescriptionRabbit polyclonal to M13 + Fd Bacteriophage Coat Proteins
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Host speciesRabbit
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Tested applicationsSuitable for: Indirect ELISA, ELISA, ICC/IFmore details
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Immunogen
fd bacteriophage
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General notesConcentration: 5.5 mg/ml- 7.5 mg/ml
Can be used as a capture antibody when coated directly on microtiter plates, or as a primary detection antibody for specifically captured M13 or fd phage. May be useful in rapidly sorting large phage display libraries (antibody, peptide etc.) with the expressed proteins fused to either the gene III or gene VIII protein of the filamentous phage. May be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
PurityIgG fraction
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Primary antibody notesCan be used as a capture antibody when coated directly on microtiter plates, or as a primary detection antibody for specifically captured M13 or fd phage. May be useful in rapidly sorting large phage display libraries (antibody, peptide etc.) with the expressed proteins fused to either the gene III or gene VIII protein of the filamentous phage. May be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab6188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Indirect ELISA | Use at an assay dependent concentration. At least 1/1000 for 5 x107 phage/mlat least 1/8000 for 5 x 1010 phage/ml. | |
| ELISA | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 17178915 |
Target
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RelevanceM13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends.
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Alternative names
- Coat protein A antibody
- Coat protein B antibody
- Coat protein C polypeptide I antibody
see all
References
This product has been referenced in:
- Phipps ML et al. Beyond Helper Phage: Using "Helper Cells" to Select Peptide Affinity Ligands. PLoS One 11:e0160940 (2016). Read more (PubMed: 27626637) »
- Vargas-Sanchez K et al. DNA Subtraction of In Vivo Selected Phage Repertoires for Efficient Peptide Pathology Biomarker Identification in Neuroinflammation Multiple Sclerosis Model. Biomark Insights 11:19-29 (2016). Read more (PubMed: 26917946) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"