Overview

  • Product name
    Anti-M13 + Fd Bacteriophage Coat Proteins antibody
  • Description
    Rabbit polyclonal to M13 + Fd Bacteriophage Coat Proteins
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Indirect ELISA, ELISA, ICC/IFmore details
  • Immunogen

    fd bacteriophage

  • General notes
    Concentration: 5.5 mg/ml- 7.5 mg/ml


    Can be used as a capture antibody when coated directly on microtiter plates, or as a primary detection antibody for specifically captured M13 or fd phage. May be useful in rapidly sorting large phage display libraries (antibody, peptide etc.) with the expressed proteins fused to either the gene III or gene VIII protein of the filamentous phage. May be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Primary antibody notes
    Can be used as a capture antibody when coated directly on microtiter plates, or as a primary detection antibody for specifically captured M13 or fd phage. May be useful in rapidly sorting large phage display libraries (antibody, peptide etc.) with the expressed proteins fused to either the gene III or gene VIII protein of the filamentous phage. May be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent concentration. At least 1/1000 for 5 x107 phage/mlat least 1/8000 for 5 x 1010 phage/ml.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 17178915

Target

  • Relevance
    M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends.
  • Alternative names
    • Coat protein A antibody
    • Coat protein B antibody
    • Coat protein C polypeptide I antibody
    • Coat protein C polypeptide II antibody
    • Coat protein D antibody
    • M13 coat protein A antibody
    • M13 coat protein B antibody
    • M13 coat protein C polypeptide I antibody
    • M13 coat protein C polypeptide II antibody
    • M13 coat protein D antibody
    • M13 minor coat protein antibody
    • Minor coat protein antibody
    see all

References

This product has been referenced in:
  • Phipps ML  et al. Beyond Helper Phage: Using "Helper Cells" to Select Peptide Affinity Ligands. PLoS One 11:e0160940 (2016). Read more (PubMed: 27626637) »
  • Vargas-Sanchez K  et al. DNA Subtraction of In Vivo Selected Phage Repertoires for Efficient Peptide Pathology Biomarker Identification in Neuroinflammation Multiple Sclerosis Model. Biomark Insights 11:19-29 (2016). Read more (PubMed: 26917946) »
See all 8 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Application
ELISA
Sample
Bacteria Recombinant protein (filamentous bacteriophage from host E. coli strain)
Specification
filamentous bacteriophage from host E. coli strain
Blocking step
BSA as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Type
Indirect

Mr. Chang Su Jeon

Verified customer

Submitted Jul 27 2015

Question
Answer

Unfortunately lot 861626 is an individual batch which was not produced from a parent batch, also I'm afraid that we no longer have stock of this batch in Europe or the USA. Sorry that I don't have better news; if you have any further questions please do not hesitate to contact me.

Read More

Answer

Thank you very much for your inquiry. The immunogen for this polyclonal antibody was a cell preparation of fd bacteriophage. We therefore do not know what epitope is bound exactly by this antibody. Therefore we also cannot make any predictions about the possible mimotopes (A mimotope is a macromolecule which mimics the structure of an epitope. Because of this property it causes an antibody response identical to the one elicited by the epitope.) that are recognized by this antibody. I am sorry I could not be of more help on this occasion and hope this information was nevertheless helpful. Please do not hesitate to contact me again with any further questions.

Read More

Answer

Thank you for contacting us. We have two anti-M13 antibodies that may be of interest: ab6188 Anti-M13 + Fd Bacteriophage Coat Proteins antibody Binds specifically to phage coat proteins of M13 or fd phage. Click here (or use the following: https://www.abcam.com/index.html?datasheet=6188). ab50370 Anti-M13 antibody [B62-FE2] (HRP) Binds to an epitope on pVIII (phage coat protein) covering the N-Terminal region of the g8p AEGDDPAKAAFDSLQASAT. Click here (or use the following: https://www.abcam.com/index.html?datasheet=50370). We have a number of HRP kits, mainly for IHC. Here is a link to a HRP substrate than can be used for WB: ab5801 Chemiluminescent HRP Substrate (membrane) Click here (or use the following: https://www.abcam.com/index.html?datasheet=5801). Please let me know if there is anything else I can help you with.

Read More

Question
Answer

Thank you for your reply. We have a number of antibodies to Bacteriophage Proteins such as ab6188: Anti-M13 + Fd Bacteriophage Coat Proteins antibody or ab9225: Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2]. I hope this helps, please let me know if you need any additional information.

Read More
Application
ELISA
Sample
Rabbit Recombinant protein (phage coat proteins of M13)
Specification
phage coat proteins of M13
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 27°C
Type
Sandwich (Capture)

Miss. I-Hsuan Chen

Verified customer

Submitted Jul 09 2010

Answer

Thank you for your enquiry. I have been in correspondence with the source of this antibody. Following discussion we concluded that there may be a degree of contamination in your samples. There is clear specific reactivity with your M13- cells. Can you tell me whether it is a possibility that this may have happened? This is supported by the fact that there is variable fluorescence in the cells, possibly reflective of the variable copy number in the M13 infection. I consider it unlikely that this antibody is demonstrating reactivity with non-specific E.coli proteins. I appreciate your comments.

Read More

Answer

Thank you for your enquiry. We have not tested the stability of the antibody ab6188 when stored in other buffer than its storage buffer so we do not know if it will improve or damage its storage conditions. In my experience it is best to use glycerol (final volume 50%) if you really wish to dilute and protect it. I hope this information helps you,

Read More

Answer

Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards.

Read More

Answer

I apologise for taking so long I have been awaiting confirmation of the concentration. The concentration of the product is batch dependent and will range from 5.5 mg/ml to 7.5 mg/ml. I hope that this information is useful to you. If I can be of any further assistance please let me know.

Read More

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up