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  1. Link

    m13--fd-bacteriophage-coat-proteins-antibody-ab6188.pdf

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Microbiology Organism Virus DNA Virus single stranded DNA Virus Enterobacteria phage M13
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Anti-M13 + Fd Bacteriophage Coat Proteins antibody (ab6188)

  • Datasheet
  • SDS
Reviews (2)Q&A (9)References (10)

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Key features and details

  • Rabbit polyclonal to M13 + Fd Bacteriophage Coat Proteins
  • Suitable for: Indirect ELISA, ELISA, ICC/IF
  • Isotype: IgG

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Overview

  • Product name

    Anti-M13 + Fd Bacteriophage Coat Proteins antibody
  • Description

    Rabbit polyclonal to M13 + Fd Bacteriophage Coat Proteins
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Indirect ELISA, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Tissue, cells or virus corresponding to M13 + Fd Bacteriophage Coat Proteins. fd bacteriophage

  • General notes

    Concentration: 5.5 mg/ml- 7.5 mg/ml


    Can be used as a capture antibody when coated directly on microtiter plates, or as a primary detection antibody for specifically captured M13 or fd phage. May be useful in rapidly sorting large phage display libraries (antibody, peptide etc.) with the expressed proteins fused to either the gene III or gene VIII protein of the filamentous phage. May be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity

    IgG fraction
  • Primary antibody notes

    Can be used as a capture antibody when coated directly on microtiter plates, or as a primary detection antibody for specifically captured M13 or fd phage. May be useful in rapidly sorting large phage display libraries (antibody, peptide etc.) with the expressed proteins fused to either the gene III or gene VIII protein of the filamentous phage. May be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • single stranded DNA Virus
    • Enterobacteria phage M13

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA
Use at an assay dependent concentration. At least 1/1000 for 5 x107 phage/mlat least 1/8000 for 5 x 1010 phage/ml.
ELISA (2)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration. PubMed: 17178915
Notes
Indirect ELISA
Use at an assay dependent concentration. At least 1/1000 for 5 x107 phage/mlat least 1/8000 for 5 x 1010 phage/ml.
ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration. PubMed: 17178915

Target

  • Relevance

    M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends.
  • Database links

    • Entrez Gene: 927331 Other species
    • Entrez Gene: 927332 Other species
    • Entrez Gene: 927333 Other species
    • Entrez Gene: 927334 Other species
    • Entrez Gene: 927335 Other species
    • GenBank: J02451.1 Other species
    • SwissProt: P69168 Other species
    • SwissProt: P69532 Other species
    • SwissProt: P69535 Other species
    • SwissProt: P69538 Other species
    • SwissProt: P69541 Other species
    see all
  • Alternative names

    • Coat protein A antibody
    • Coat protein B antibody
    • Coat protein C polypeptide I antibody
    • Coat protein C polypeptide II antibody
    • Coat protein D antibody
    • M13 coat protein A antibody
    • M13 coat protein B antibody
    • M13 coat protein C polypeptide I antibody
    • M13 coat protein C polypeptide II antibody
    • M13 coat protein D antibody
    • M13 minor coat protein antibody
    • Minor coat protein antibody
    see all

Protocols

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (10)

Publishing research using ab6188? Please let us know so that we can cite the reference in this datasheet.

ab6188 has been referenced in 10 publications.

  • Fraser BA  et al. A novel approach to nonsurgical sterilization; application of menadione-modified gonocyte-targeting M13 bacteriophage for germ cell ablation in utero. Pharmacol Res Perspect 8:e00654 (2020). PubMed: 32930516
  • Li X  et al. A novel peptide targets CD105 for tumour imaging in vivo. Oncol Rep 40:2935-2943 (2018). PubMed: 30132552
  • Phipps ML  et al. Beyond Helper Phage: Using "Helper Cells" to Select Peptide Affinity Ligands. PLoS One 11:e0160940 (2016). PubMed: 27626637
  • Vargas-Sanchez K  et al. DNA Subtraction of In Vivo Selected Phage Repertoires for Efficient Peptide Pathology Biomarker Identification in Neuroinflammation Multiple Sclerosis Model. Biomark Insights 11:19-29 (2016). PubMed: 26917946
  • Costantini TW  et al. Intravenous phage display identifies peptide sequences that target the burn-injured intestine. Peptides 38:94-9 (2012). PubMed: 22960048
  • Wu D  et al. Phage Displayed Peptides to Avian H5N1 Virus Distinguished the Virus from Other Viruses. PLoS One 6:e23058 (2011). ELISA . PubMed: 21887228
  • Antonara S  et al. Borrelia burgdorferi adhesins identified using in vivo phage display. Mol Microbiol 66:262-76 (2007). ELISA . PubMed: 17784908
  • Kelly KA  et al. SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration. J Leukoc Biol 81:748-56 (2007). ICC/IF . PubMed: 17178915
  • Hekim C  et al. Novel peptide inhibitors of human kallikrein 2. J Biol Chem 281:12555-60 (2006). PubMed: 16527822
  • Kelly KA  et al. Detection of vascular adhesion molecule-1 expression using a novel multimodal nanoparticle. Circ Res 96:327-36 (2005). PubMed: 15653572

Customer reviews and Q&As

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1-10 of 11 Abreviews or Q&A

ELISA abreview for Anti-M13 + Fd Bacteriophage Coat Proteins antibody

Excellent
Abreviews
Abreviews
abreview image
Application
ELISA
Sample
Bacteria Recombinant protein (filamentous bacteriophage from host E. coli strain)
Specification
filamentous bacteriophage from host E. coli strain
Blocking step
BSA as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Type
Indirect
Read More

Mr. Chang Su Jeon

Verified customer

Submitted Jul 27 2015

ELISA abreview for Anti-M13 + Fd Bacteriophage Coat Proteins antibody

Excellent
Abreviews
Abreviews
Application
ELISA
Sample
Rabbit Recombinant protein (phage coat proteins of M13)
Specification
phage coat proteins of M13
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 27°C
Type
Sandwich (Capture)
Read More

Miss. I-Hsuan Chen

Verified customer

Submitted Jul 09 2010

Question

I would like to get more vials of lot 861626. How many more are available?

Read More

Abcam community

Verified customer

Asked on Dec 16 2011

Answer

Unfortunately lot 861626 is an individual batch which was not produced from a parent batch, also I'm afraid that we no longer have stock of this batch in Europe or the USA. Sorry that I don't have better news; if you have any further questions please do not hesitate to contact me.

Read More

Abcam Scientific Support

Answered on Dec 16 2011

Question

 Hi, I am wondering if peptide mimotope of your Anti-M13 + Fd Bacteriophage Coat Proteins antibody is known?

Read More

Abcam community

Verified customer

Asked on Oct 31 2011

Answer

Thank you very much for your inquiry. The immunogen for this polyclonal antibody was a cell preparation of fd bacteriophage. We therefore do not know what epitope is bound exactly by this antibody. Therefore we also cannot make any predictions about the possible mimotopes (A mimotope is a macromolecule which mimics the structure of an epitope. Because of this property it causes an antibody response identical to the one elicited by the epitope.) that are recognized by this antibody. I am sorry I could not be of more help on this occasion and hope this information was nevertheless helpful. Please do not hesitate to contact me again with any further questions.

Read More

Abcam Scientific Support

Answered on Oct 31 2011

Question

Thank you very much for reply. So do you supply the anti-pVIII protein of M13 phage antibody for ELISA (used in phage display experiment)? And HRP/AntiM13 and HRP substrate OPD (O-phenyldiamine dihydrochloride)?   Thank you very much.  

Read More

Abcam community

Verified customer

Asked on Sep 15 2011

Answer

Thank you for contacting us. We have two anti-M13 antibodies that may be of interest: ab6188 Anti-M13 + Fd Bacteriophage Coat Proteins antibody Binds specifically to phage coat proteins of M13 or fd phage. Click here (or use the following: https://www.abcam.com/M13--Fd-Bacteriophage-Coat-Proteins-antibody-ab6188.html). ab50370 Anti-M13 antibody [B62-FE2] (HRP) Binds to an epitope on pVIII (phage coat protein) covering the N-Terminal region of the g8p AEGDDPAKAAFDSLQASAT. Click here (or use the following: https://www.abcam.com/HRP-M13-antibody-B62-FE2-ab50370.html). We have a number of HRP kits, mainly for IHC. Here is a link to a HRP substrate than can be used for WB: ab5801 Chemiluminescent HRP Substrate (membrane) Click here (or use the following: https://www.abcam.com/Chemiluminescent-HRP-Substrate-membrane-ab5801.html). Please let me know if there is anything else I can help you with.

Read More

Abcam Scientific Support

Answered on Sep 15 2011

Question

Do you have antibodies for phage proteins?

Read More

Abcam community

Verified customer

Asked on Sep 14 2011

Answer

Thank you for your reply. We have a number of antibodies to Bacteriophage Proteins such as ab6188: Anti-M13 + Fd Bacteriophage Coat Proteins antibody or ab9225: Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2]. I hope this helps, please let me know if you need any additional information.

Read More

Abcam Scientific Support

Answered on Sep 14 2011

Question

I have a question regarding antibody ab6188 (M13 bacteriophage coat proteins). We used this antibody in immunofluorescence stainings and observed an intense cross-reactivity with (non M13-infected) E. coli, apparently with one of the outer membrane proteins. In fact, it was the major signal (see attached pictures). We used the antibody in a 1:50 dilution in PBS and used skim milk powder as blocking reagent. Detection was with an Alexa Fluor 488 - coupled secondary antibody. A negative control (ab6188 omitted) resulted in zero fluorescence, as expected. As we want to use the antibody in part to discriminate between infected and non-infected E. coli, this is clearly unacceptable. Do you have any suggestion how to solve this problem?

Read More

Abcam community

Verified customer

Asked on Nov 16 2006

Answer

Thank you for your enquiry. I have been in correspondence with the source of this antibody. Following discussion we concluded that there may be a degree of contamination in your samples. There is clear specific reactivity with your M13- cells. Can you tell me whether it is a possibility that this may have happened? This is supported by the fact that there is variable fluorescence in the cells, possibly reflective of the variable copy number in the M13 infection. I consider it unlikely that this antibody is demonstrating reactivity with non-specific E.coli proteins. I appreciate your comments.

Read More

Abcam Scientific Support

Answered on Nov 17 2006

Question

I have your ab6188 antibody. Was wondering if aliquoting with ethylene glycol will improve its life?

Read More

Abcam community

Verified customer

Asked on Nov 01 2005

Answer

Thank you for your enquiry. We have not tested the stability of the antibody ab6188 when stored in other buffer than its storage buffer so we do not know if it will improve or damage its storage conditions. In my experience it is best to use glycerol (final volume 50%) if you really wish to dilute and protect it. I hope this information helps you,

Read More

Abcam Scientific Support

Answered on Nov 02 2005

Question

I have technical difficult with the other anti-phage M13 antibodies and I was wondering if I can have a samples of your antibody to test it for immunofluorescence staining in frozen tissues if this has not been done before.

Read More

Abcam community

Verified customer

Asked on Mar 28 2005

Answer

Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards.

Read More

Abcam Scientific Support

Answered on Mar 28 2005

Question

Can you please tell me the concentration of your 'M13 Bacteriophage Coat Proteins' antibody #AB6188? It is an IgG fraction, so I hope it has been measured. Kind regards,

Read More

Abcam community

Verified customer

Asked on Aug 11 2004

Answer

I apologise for taking so long I have been awaiting confirmation of the concentration. The concentration of the product is batch dependent and will range from 5.5 mg/ml to 7.5 mg/ml. I hope that this information is useful to you. If I can be of any further assistance please let me know.

Read More

Abcam Scientific Support

Answered on Aug 19 2004

1-10 of 11 Abreviews or Q&A

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