Anti-M13 + Fd Bacteriophage Coat Proteins antibody (ab6188)

Unfortunately lot 861626 is an individual batch which was not produced from a parent batch, also I'm afraid that we no longer have stock of this batch in Europe or the USA. Sorry that I don't have better news; if you have any further questions please do not hesitate to contact me.

Thank you very much for your inquiry. The immunogen for this polyclonal antibody was a cell preparation of fd bacteriophage. We therefore do not know what epitope is bound exactly by this antibody. Therefore we also cannot make any predictions about the possible mimotopes (A mimotope is a macromolecule which mimics the structure of an epitope. Because of this property it causes an antibody response identical to the one elicited by the epitope.) that are recognized by this antibody. I am sorry I could not be of more help on this occasion and hope this information was nevertheless helpful. Please do not hesitate to contact me again with any further questions.

Thank you for contacting us. We have two anti-M13 antibodies that may be of interest: ab6188 Anti-M13 + Fd Bacteriophage Coat Proteins antibody Binds specifically to phage coat proteins of M13 or fd phage. Click here (or use the following: https://www.abcam.com/M13--Fd-Bacteriophage-Coat-Proteins-antibody-ab6188.html). ab50370 Anti-M13 antibody [B62-FE2] (HRP) Binds to an epitope on pVIII (phage coat protein) covering the N-Terminal region of the g8p AEGDDPAKAAFDSLQASAT. Click here (or use the following: https://www.abcam.com/HRP-M13-antibody-B62-FE2-ab50370.html). We have a number of HRP kits, mainly for IHC. Here is a link to a HRP substrate than can be used for WB: ab5801 Chemiluminescent HRP Substrate (membrane) Click here (or use the following: https://www.abcam.com/Chemiluminescent-HRP-Substrate-membrane-ab5801.html). Please let me know if there is anything else I can help you with.

Thank you for your reply. We have a number of antibodies to Bacteriophage Proteins such as ab6188: Anti-M13 + Fd Bacteriophage Coat Proteins antibody or ab9225: Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2]. I hope this helps, please let me know if you need any additional information.

Thank you for your enquiry. I have been in correspondence with the source of this antibody. Following discussion we concluded that there may be a degree of contamination in your samples. There is clear specific reactivity with your M13- cells. Can you tell me whether it is a possibility that this may have happened? This is supported by the fact that there is variable fluorescence in the cells, possibly reflective of the variable copy number in the M13 infection. I consider it unlikely that this antibody is demonstrating reactivity with non-specific E.coli proteins. I appreciate your comments.

Thank you for your enquiry. We have not tested the stability of the antibody ab6188 when stored in other buffer than its storage buffer so we do not know if it will improve or damage its storage conditions. In my experience it is best to use glycerol (final volume 50%) if you really wish to dilute and protect it. I hope this information helps you,

Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards.

I apologise for taking so long I have been awaiting confirmation of the concentration. The concentration of the product is batch dependent and will range from 5.5 mg/ml to 7.5 mg/ml. I hope that this information is useful to you. If I can be of any further assistance please let me know.