Anti-M13 antibody [E1] (ab24229)
Mouse monoclonal M13 antibody [E1]. Validated in WB, ELISA. Independently reviewed in 2 review(s).
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-M13 antibody [E1]
See all M13 primary antibodies -
Description
Mouse monoclonal [E1] to M13 -
Host species
Mouse -
Specificity
Antibody specific for gp3 protein. -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
M13 bacteriophage.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.08% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purification notes
Purified over protein A/G column. -
Clonality
Monoclonal -
Clone number
E1 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab24229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| ELISA |
Application notes
ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 45 kDa.
Sorting of M13 infected cells.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 45 kDa.
Sorting of M13 infected cells.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
The bacteriophage known as "M13" forms the basis of cloning systems designed to easily introduce mutations into genes inserted into the phage genome. It also has been used in various "phage display" methodologies and combinatorial DNA and peptide libraries. Coat protein A (G3P) is necessary for adsorption of the virion onto the F-pilus of the host cell. There are about five copies of this protein per mature phage. Consists of three domains (N1, N2, and CT). The N2 domain interacts with the F pilus, whereas the N1 domain (connected to N2 by a flexible glycine-rich linker and tightly interacting with it on the phage) forms a complex with the C-terminal domain of tolA at later stages of the infection process. They are located at the adsorption end of the phage particle. -
Alternative names
- Bacteriophage M13 antibody
- Coliphage M13 antibody
- Enterobacteria phage M13 antibody
see all
Protocols
References
This product has been referenced in:
- Wang L et al. An ultra-sensitive capacitive microwire sensor for pathogen-specific serum antibody responses. Biosens Bioelectron 131:46-52 (2019). Read more (PubMed: 30822687) »
- Yamamoto M et al. Efficacy of Tumor-Targeting Salmonella A1-R on a Melanoma Patient-Derived Orthotopic Xenograft (PDOX) Nude-Mouse Model. PLoS One 11:e0160882 (2016). Read more (PubMed: 27500926) »
