Anti-M13 antibody [E1] (ab24229)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-M13 antibody [E1]
See all M13 primary antibodies -
DescriptionMouse monoclonal [E1] to M13
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Host speciesMouse
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SpecificityAntibody specific for gp3 protein.
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Tested applicationsSuitable for: WB, ELISAmore details
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Species reactivityReacts with: Species independent
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Immunogen
M13 bacteriophage.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.08% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purification notesPurified over protein A/G column.
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ClonalityMonoclonal
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Clone numberE1
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IsotypeIgG2a
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab24229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| ELISA |
Application notes
ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 45 kDa.
Sorting of M13 infected cells.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 45 kDa.
Sorting of M13 infected cells.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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RelevanceThe bacteriophage known as "M13" forms the basis of cloning systems designed to easily introduce mutations into genes inserted into the phage genome. It also has been used in various "phage display" methodologies and combinatorial DNA and peptide libraries. Coat protein A (G3P) is necessary for adsorption of the virion onto the F-pilus of the host cell. There are about five copies of this protein per mature phage. Consists of three domains (N1, N2, and CT). The N2 domain interacts with the F pilus, whereas the N1 domain (connected to N2 by a flexible glycine-rich linker and tightly interacting with it on the phage) forms a complex with the C-terminal domain of tolA at later stages of the infection process. They are located at the adsorption end of the phage particle.
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Alternative names
- Bacteriophage M13 antibody
- Coliphage M13 antibody
- Enterobacteria phage M13 antibody
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Protocols
References
ab24229 has not yet been referenced specifically in any publications.
