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  1. Link

    m13-antibody-e1-ab24229.pdf

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Microbiology Organism Virus DNA Virus single stranded DNA Virus Enterobacteria phage M13
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Anti-M13 antibody [E1] (ab24229)

  • Datasheet
  • SDS
Reviews (2)Q&A (2)References (2)

Product price, shipping and contact information

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Key features and details

  • Mouse monoclonal [E1] to M13
  • Suitable for: WB, ELISA
  • Reacts with: Species independent
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-M13 antibody [E1]
    See all M13 primary antibodies
  • Description

    Mouse monoclonal [E1] to M13
  • Host species

    Mouse
  • Specificity

    Antibody specific for gp3 protein.
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Tissue, cells or virus corresponding to M13. Hybridoma produced by the fusion of splenocytes from mice immunized with isolated M13 phage coat proteins and mouse myeloma cells

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.08% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purification notes

    Purified over protein A/G column.
  • Clonality

    Monoclonal
  • Clone number

    E1
  • Isotype

    IgG2a
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • single stranded DNA Virus
    • Enterobacteria phage M13

Associated products

  • Alternative Versions

    • Biotin Anti-M13 antibody [E1] (ab17269)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab24229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 45 kDa.

Native conditions may be required.

ELISA (1)
Use at an assay dependent concentration.
Notes
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 45 kDa.

Native conditions may be required.

ELISA
Use at an assay dependent concentration.

Target

  • Relevance

    The bacteriophage known as "M13" forms the basis of cloning systems designed to easily introduce mutations into genes inserted into the phage genome. It also has been used in various "phage display" methodologies and combinatorial DNA and peptide libraries. Coat protein A (G3P) is necessary for adsorption of the virion onto the F-pilus of the host cell. There are about five copies of this protein per mature phage. Consists of three domains (N1, N2, and CT). The N2 domain interacts with the F pilus, whereas the N1 domain (connected to N2 by a flexible glycine-rich linker and tightly interacting with it on the phage) forms a complex with the C-terminal domain of tolA at later stages of the infection process. They are located at the adsorption end of the phage particle.
  • Alternative names

    • Bacteriophage M13 antibody
    • Coliphage M13 antibody
    • Enterobacteria phage M13 antibody
    • M13 virus antibody
    • Phage M13 antibody
    see all

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab24229? Please let us know so that we can cite the reference in this datasheet.

ab24229 has been referenced in 2 publications.

  • Wang L  et al. An ultra-sensitive capacitive microwire sensor for pathogen-specific serum antibody responses. Biosens Bioelectron 131:46-52 (2019). PubMed: 30822687
  • Yamamoto M  et al. Efficacy of Tumor-Targeting Salmonella A1-R on a Melanoma Patient-Derived Orthotopic Xenograft (PDOX) Nude-Mouse Model. PLoS One 11:e0160882 (2016). PubMed: 27500926

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-4 of 4 Abreviews or Q&A

ELISA abreview for Anti-M13 antibody [E1]

Excellent
Abreviews
Abreviews
abreview image
Application
ELISA
Sample
Bacteria Recombinant protein (filamentous bacteriophage from host E. coli strain)
Specification
filamentous bacteriophage from host E. coli strain
Blocking step
BSA as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Type
Indirect
Read More

Mr. Chang Su Jeon

Verified customer

Submitted Aug 13 2015

Immunocytochemistry abreview for Anti-M13 antibody [E1]

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Bacteria Cultured Cells (Phage Suspension)
Permeabilization
No
Specification
Phage Suspension
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
none
Read More

Abcam user community

Verified customer

Submitted Oct 06 2015

Question

I want to do an ELISA with whole phage but I want it to specifically target gp3 protein on whole phage. Do you know if your antibody recognizes an epitope that is exposed in whole phage?

Read More

Abcam community

Verified customer

Asked on May 21 2013

Answer

Yes, the antibody does detect intact phage, at the gp3 protein.

Read More

Abcam Scientific Support

Answered on May 21 2013

Question

ab24229 M13 antibody [E1] Do you have any epitope information for this product i.e. the domain that the antibody recognizes?

Read More

Abcam community

Verified customer

Asked on Jun 07 2007

Answer

Thank you for your enquiry. Regarding ab24229 (M13 antibody [E1]), this antibody is specific for p3 but we do not have more epitope information at this time.

Read More

Abcam Scientific Support

Answered on Jun 07 2007

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