Key features and details
- Mouse monoclonal [E1] to M13
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Isotype: IgG2a
Product nameAnti-M13 antibody [E1]
See all M13 primary antibodies
DescriptionMouse monoclonal [E1] to M13
SpecificityAntibody specific for gp3 protein.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Species independent
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.08% Sodium azide
Concentration information loading...
Purification notesPurified over protein A/G column.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 45 kDa.
Sorting of M13 infected cells.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceThe bacteriophage known as "M13" forms the basis of cloning systems designed to easily introduce mutations into genes inserted into the phage genome. It also has been used in various "phage display" methodologies and combinatorial DNA and peptide libraries. Coat protein A (G3P) is necessary for adsorption of the virion onto the F-pilus of the host cell. There are about five copies of this protein per mature phage. Consists of three domains (N1, N2, and CT). The N2 domain interacts with the F pilus, whereas the N1 domain (connected to N2 by a flexible glycine-rich linker and tightly interacting with it on the phage) forms a complex with the C-terminal domain of tolA at later stages of the infection process. They are located at the adsorption end of the phage particle.
- Bacteriophage M13 antibody
- Coliphage M13 antibody
- Enterobacteria phage M13 antibody
ab24229 has been referenced in 2 publications.
- Wang L et al. An ultra-sensitive capacitive microwire sensor for pathogen-specific serum antibody responses. Biosens Bioelectron 131:46-52 (2019). PubMed: 30822687
- Yamamoto M et al. Efficacy of Tumor-Targeting Salmonella A1-R on a Melanoma Patient-Derived Orthotopic Xenograft (PDOX) Nude-Mouse Model. PLoS One 11:e0160882 (2016). PubMed: 27500926