Overview

  • Product name

    Anti-M13 antibody [E1] (Biotin)
    See all M13 primary antibodies
  • Description

    Mouse monoclonal [E1] to M13 (Biotin)
  • Host species

    Mouse
  • Conjugation

    Biotin
  • Specificity

    M13 antibody [E1] (ab17269) is specific for gp3 protein.
  • Tested applications

    Suitable for: WB, ELISA, Flow Cytmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Full length M13 phage coat protein; Exact epitope not determined.

  • General notes


    M13 antibody [E1] (ab17269) has numerous uses in screening phage display libraries, especially the screening of single chain antibody libraries.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.08% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    M13 antibody [E1] (ab17269) is purified using 0.2µm sterile filter.
  • Primary antibody notes

    M13 antibody [E1] (ab17269) has numerous uses in screening phage display libraries, especially the screening of single chain antibody libraries.
  • Clonality

    Monoclonal
  • Clone number

    E1
  • Myeloma

    unknown
  • Isotype

    IgG3
  • Light chain type

    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 90 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab97679 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance

    The bacteriophage known as "M13" forms the basis of cloning systems designed to easily introduce mutations into genes inserted into the phage genome. It also has been used in various "phage display" methodologies and combinatorial DNA and peptide libraries. Coat protein A (G3P) is necessary for adsorption of the virion onto the F-pilus of the host cell. There are about five copies of this protein per mature phage. Consists of three domains (N1, N2, and CT). The N2 domain interacts with the F pilus, whereas the N1 domain (connected to N2 by a flexible glycine-rich linker and tightly interacting with it on the phage) forms a complex with the C-terminal domain of tolA at later stages of the infection process. They are located at the adsorption end of the phage particle.
  • Alternative names

    • Bacteriophage M13 antibody
    • Coliphage M13 antibody
    • Enterobacteria phage M13 antibody
    • M13 virus antibody
    • Phage M13 antibody
    see all

References

ab17269 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab17269.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up