Overview

  • Product name

    Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2]
  • Description

    Mouse monoclonal [RL-ph2] to M13 Bacteriophage Coat Protein g8p
  • Host species

    Mouse
  • Specificity

    This antibody reacts with the M13 filamentous phage coat protein g8p.
  • Tested applications

    Suitable for: ICC, Flow Cyt, ELISA, WBmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Isolated M13 phage coat proteins

  • General notes


    The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Primary antibody notes

    The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.
  • Clonality

    Monoclonal
  • Clone number

    RL-ph2
  • Myeloma

    Sp2/0-Ag14
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
Flow Cyt 1/25 - 1/200.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ELISA Use at an assay dependent concentration.
WB 1/100 - 1/1000. Detects a band of approximately 5 kDa (predicted molecular weight: 5 kDa).

Target

  • Relevance

    M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. The minor coat protein P3 attaches to the receptor at the tip of the F pilus of the host Escherichia coli. Infection with filamentous phages is not lethal, however the infection causes turbid plaques in E. coli. It is a non-lytic virus. However a decrease in the rate of cell growth is seen in the infected cells. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.
  • Database links

    • Alternative names

      • Capsid protein G8P antibody
      • Coat protein B antibody
      • G8P antibody
      • Major coat protein antibody
      • VIII antibody
      see all

    Images

    • Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225) at 1/1000 dilution + whole cell lysate prepared from XL10Gold cells at 50 µg

      Secondary
      Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/2000 dilution

      Predicted band size: 5 kDa
      Observed band size: 6 kDa
      why is the actual band size different from the predicted?



      Primary antibody incubated for 16 hours at 4°C.
      Blocking step performed using 5% milk for 1 hour at room temperature.

      See Abreview

    References

    This product has been referenced in:

    • Meulemans EV  et al. Phage displayed antibodies specific for a cytoskeletal antigen. Selection by competitive elution with a monoclonal antibody. Hum Antibodies Hybridomas 6:113-8 (1995). Read more (PubMed: 8597626) »
    • Meulemans EV  et al. Selection of phage-displayed antibodies specific for a cytoskeletal antigen by competitive elution with a monoclonal antibody. J Mol Biol 244:353-60 (1994). Read more (PubMed: 7527464) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-8 of 8 Abreviews or Q&A

    Answer

    Thank you for your enquiry and your interest. Currently, we only have antibodies against M13 Bacteriophage Coat Protein g8p but not the purified protein. If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Question
    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number ***.
    To check the status of the order please contact our Customer Service team and reference this number.
    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
    I wish you the best of luck with your research.

    Read More

    Question
    Answer

    Thank you for contacting Abcam.

    I am sorry to hear of the issues that you have been experimenting with this latest lot of ab9225. I have had the opportunity to speak with my collegues about adding the lateral flow application to our Abreview program. Unfortunately this will not be possible at this time.

    As I mentioned on the phone, this product has not been tested in the type of assay that you have been performing and as such we cannot guarantee that it will give satifactor results from lot to lot. I am sorry for any difficulties that this has caused.

    Please let me know if there if anything else that we may do to help you meet your research goals.

    Read More

    Answer

    Thank you for contacting us. Unfortunately we currently do not have any M13 Bacteriophage Coat g8p protein in our catalogue. I'm sorry I could not have been of more help but if you have any further questions please do not hesitate to ask.

    Read More

    Question
    Answer

    Thank you for your reply. We have a number of antibodies to Bacteriophage Proteins such as ab6188: Anti-M13 + Fd Bacteriophage Coat Proteins antibody or ab9225: Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2]. I hope this helps, please let me know if you need any additional information.

    Read More
    Application
    Western blot
    Sample
    Escherichia coli Cell lysate - whole cell (XL10Gold)
    Loading amount
    50 µg
    Specification
    XL10Gold
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Sep 20 2010

    Application
    ELISA
    Sample
    Escherichia coli Cell (XL10Gold)
    Specification
    XL10Gold
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Type
    Direct

    Abcam user community

    Verified customer

    Submitted Apr 07 2009

    Answer

    Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will forward a GBP10/ USD15/ EUR15 Amazon gift voucher.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up