Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225)
![Western blot - Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225)](https://www.abcam.com/ps/products/9/ab9225/Images/ab9225-16856-anti-m13-bacteriophage-coat-protein-g8p-antibody-rl-ph2-western-blot.jpg "Western blot - Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225)")
Key features and details
- Mouse monoclonal [RL-ph2] to M13 Bacteriophage Coat Protein g8p
- Suitable for: ICC, Flow Cyt, WB
- Isotype: IgG2a
Overview
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Product name
Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] -
Description
Mouse monoclonal [RL-ph2] to M13 Bacteriophage Coat Protein g8p -
Host species
Mouse -
Specificity
RL-ph2 reacts with the major M13 filamentous phage coat protein g8p with a molecular weight of 5 kDa.
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Tested applications
Suitable for: ICC, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Recombinant full length protein corresponding to M13 Bacteriophage Coat Protein g8p. Isolated M13 phage coat proteins
Database link: P69541 -
General notes
The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Primary antibody notes
The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. -
Clonality
Monoclonal -
Clone number
RL-ph2 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
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| Flow Cyt |
1/25 - 1/200.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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| WB | (1) |
1/100 - 1/1000. Detects a band of approximately 5 kDa (predicted molecular weight: 5 kDa).
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| Notes |
|---|
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ICC
Use at an assay dependent concentration. |
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Flow Cyt
1/25 - 1/200. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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WB
1/100 - 1/1000. Detects a band of approximately 5 kDa (predicted molecular weight: 5 kDa). |
Target
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Relevance
M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. The minor coat protein P3 attaches to the receptor at the tip of the F pilus of the host Escherichia coli. Infection with filamentous phages is not lethal, however the infection causes turbid plaques in E. coli. It is a non-lytic virus. However a decrease in the rate of cell growth is seen in the infected cells. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. -
Database links
- Entrez Gene: 927333 Other species
- SwissProt: P69541 Other species
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Alternative names
- Capsid protein G8P antibody
- Coat protein B antibody
- G8P antibody
see all
Images
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Western blot - Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225)Image courtesy of an anonymous Abreview.Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225) at 1/1000 dilution + whole cell lysate prepared from XL10Gold cells at 50 µg
Secondary
Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/2000 dilution
Predicted band size: 5 kDa
Observed band size: 6 kDa why is the actual band size different from the predicted?
Primary antibody incubated for 16 hours at 4°C.Blocking step performed using 5% milk for 1 hour at room temperature.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab9225 has been referenced in 3 publications.
- Hulin-Curtis SL et al. Identification of folate receptor a (FRa) binding oligopeptides and their evaluation for targeted virotherapy applications. Cancer Gene Ther 27:785-798 (2020). PubMed: 31902944
- Meulemans EV et al. Phage displayed antibodies specific for a cytoskeletal antigen. Selection by competitive elution with a monoclonal antibody. Hum Antibodies Hybridomas 6:113-8 (1995). PubMed: 8597626
- Meulemans EV et al. Selection of phage-displayed antibodies specific for a cytoskeletal antigen by competitive elution with a monoclonal antibody. J Mol Biol 244:353-60 (1994). PubMed: 7527464