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Microbiology Organism Virus DNA Virus single stranded DNA Virus Enterobacteria phage M13
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Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225)

  • Datasheet
  • SDS
Reviews (2)Q&A (6)References (3)

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Western blot - Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225)

    Key features and details

    • Mouse monoclonal [RL-ph2] to M13 Bacteriophage Coat Protein g8p
    • Suitable for: ICC, Flow Cyt, WB
    • Isotype: IgG2a

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    Overview

    • Product name

      Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2]
    • Description

      Mouse monoclonal [RL-ph2] to M13 Bacteriophage Coat Protein g8p
    • Host species

      Mouse
    • Specificity

      RL-ph2 reacts with the major M13 filamentous phage coat protein g8p with a molecular weight of 5 kDa.

    • Tested applications

      Suitable for: ICC, Flow Cyt, WBmore details
    • Species reactivity

      Reacts with: Other species
    • Immunogen

      Recombinant full length protein corresponding to M13 Bacteriophage Coat Protein g8p. Isolated M13 phage coat proteins
      Database link: P69541

    • General notes


      The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Primary antibody notes

      The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.
    • Clonality

      Monoclonal
    • Clone number

      RL-ph2
    • Myeloma

      Sp2/0-Ag14
    • Isotype

      IgG2a
    • Light chain type

      kappa
    • Research areas

      • Microbiology
      • Organism
      • Virus
      • DNA Virus
      • single stranded DNA Virus
      • Enterobacteria phage M13

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab9225 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC
    Use at an assay dependent concentration.
    Flow Cyt
    1/25 - 1/200.

    ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

     

    WB (1)
    1/100 - 1/1000. Detects a band of approximately 5 kDa (predicted molecular weight: 5 kDa).
    Notes
    ICC
    Use at an assay dependent concentration.
    Flow Cyt
    1/25 - 1/200.

    ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

     

    WB
    1/100 - 1/1000. Detects a band of approximately 5 kDa (predicted molecular weight: 5 kDa).

    Target

    • Relevance

      M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. The minor coat protein P3 attaches to the receptor at the tip of the F pilus of the host Escherichia coli. Infection with filamentous phages is not lethal, however the infection causes turbid plaques in E. coli. It is a non-lytic virus. However a decrease in the rate of cell growth is seen in the infected cells. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.
    • Database links

      • Entrez Gene: 927333 Other species
      • SwissProt: P69541 Other species
      • Alternative names

        • Capsid protein G8P antibody
        • Coat protein B antibody
        • G8P antibody
        • Major coat protein antibody
        • VIII antibody
        see all

      Images

      • Western blot - Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225)
        Western blot - Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225)Image courtesy of an anonymous Abreview.
        Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225) at 1/1000 dilution + whole cell lysate prepared from XL10Gold cells at 50 µg

        Secondary
        Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/2000 dilution

        Predicted band size: 5 kDa
        Observed band size: 6 kDa why is the actual band size different from the predicted?



        Primary antibody incubated for 16 hours at 4°C.
        Blocking step performed using 5% milk for 1 hour at room temperature.

        See Abreview

      Protocols

      • Flow cytometry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (3)

      Publishing research using ab9225? Please let us know so that we can cite the reference in this datasheet.

      ab9225 has been referenced in 3 publications.

      • Hulin-Curtis SL  et al. Identification of folate receptor a (FRa) binding oligopeptides and their evaluation for targeted virotherapy applications. Cancer Gene Ther 27:785-798 (2020). PubMed: 31902944
      • Meulemans EV  et al. Phage displayed antibodies specific for a cytoskeletal antigen. Selection by competitive elution with a monoclonal antibody. Hum Antibodies Hybridomas 6:113-8 (1995). PubMed: 8597626
      • Meulemans EV  et al. Selection of phage-displayed antibodies specific for a cytoskeletal antigen by competitive elution with a monoclonal antibody. J Mol Biol 244:353-60 (1994). PubMed: 7527464

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review

      Filter by Application

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      1-2 of 2 Abreviews

      Western blot abreview for Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Escherichia coli Cell lysate - whole cell (XL10Gold)
      Loading amount
      50 µg
      Specification
      XL10Gold
      Gel Running Conditions
      Reduced Denaturing (4-20%)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
      Read More

      Abcam user community

      Verified customer

      Submitted Sep 20 2010

      ELISA abreview for Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      ELISA
      Sample
      Escherichia coli Cell (XL10Gold)
      Specification
      XL10Gold
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
      Type
      Direct
      Read More

      Abcam user community

      Verified customer

      Submitted Apr 07 2009

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