Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)

Overview

  • Product name

    Anti-M6PR (cation dependent) antibody [EPR7691]
    See all M6PR (cation dependent) primary antibodies
  • Description

    Rabbit monoclonal [EPR7691] to M6PR (cation dependent)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human M6PR (cation dependent) aa 250 to the C-terminus (C terminal).
    Database link: P20645

  • Positive control

    • WB: HAP1, A549, and Human uterus lysates ICC/IF: HeLa cell lysate FC: A549 cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
Flow Cyt 1/80.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex.
    • Domain

      The extracellular domain is homologous to the repeating units (of approximately 147 AA) of the cation-independent mannose 6-phosphate receptor.
    • Cellular localization

      Lysosome membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 46 kDa mannose 6 phosphate receptor antibody
      • 46 kDa mannose 6-phosphate receptor antibody
      • Cation dependent mannose 6 phosphate receptor antibody
      • Cation dependent mannose 6 phosphate receptor precursor antibody
      • Cation-dependent mannose-6-phosphate receptor antibody
      • CD Man 6 P receptor antibody
      • CD Man-6-P receptor antibody
      • CD MPR antibody
      • CD-M6PR antibody
      • CD-MPR antibody
      • FLJ32994 antibody
      • M6pr antibody
      • M6PR protein antibody
      • Man6PR antibody
      • Mannose 6 phosphate receptor (cation dependent) antibody
      • Mannose 6 phosphate receptor antibody
      • MPR 46 antibody
      • MPR46 antibody
      • MPRD antibody
      • MPRD_HUMAN antibody
      • Mr 46,000 Man6PR antibody
      • Small mannose 6 phosphate receptor antibody
      • SMPR antibody
      see all

    Images

    • Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: Empty
      Lane 3: M6PR knockout HAP1 whole cell lysate (20 µg)
      Lane 4: A549 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - unpurified ab134153 observed at 46 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab134153 was shown to specifically react with M6PR when M6PR knockout samples were used. Wild-type and M6PR knockout samples were subjected to SDS-PAGE. ab134153 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling M6PR (cation dependent) with Purified ab134153 at 1:100 dilution (8.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor®488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • All lanes : Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153) at 0.8 µg/ml (purified)

      Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
      Lane 2 : Mouse kidney lysates
      Lane 3 : Rat kidney lysates
      Lane 4 : Rat spleen lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 31 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

    • Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling M6PR (cation dependent) with purified ab134153 at 1:80 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    • All lanes : Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153) at 1/1000 dilution (Unpurified)

      Lane 1 : A549 lysates
      Lane 2 : Human uterus lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 31 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Di Stefano AL  et al. Detection, Characterization, and Inhibition of FGFR-TACC Fusions in IDH Wild-type Glioma. Clin Cancer Res 21:3307-17 (2015). Read more (PubMed: 25609060) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Question
    Answer

    Thank you for your call today and for letting us know about the trouble with ab76276.

    As we discussed, I'm sending a free of charge vial of ab134153 on the order ***, which I expect to arrive by July 9. Please keep me updated about the results with this antibody.

    I look forward to hearing from you. If there is anything else that I can dofor you in the meantime, please let me know and I'll be happy to help.

    Have a nice day.

    Read More

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