Recombinant Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
![Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)](https://www.abcam.com/ps/products/134/ab134153/Images/ab134153-275424-anti-m6pr-cation-dependent-antibody-epr7691-western-blot.jpg "Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7691] to M6PR (cation dependent)
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-M6PR (cation dependent) antibody [EPR7691]
See all M6PR (cation dependent) primary antibodies -
Description
Rabbit monoclonal [EPR7691] to M6PR (cation dependent) -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human M6PR (cation dependent) aa 250 to the C-terminus (C terminal).
Database link: P20645 -
Positive control
- WB: HAP1, A549, and Human uterus lysates ICC/IF: HeLa cell lysate Flow Cyt (intra): A549 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 6.30 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7691 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/80.
|
|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
|
| ICC/IF | (1) |
1/50 - 1/100.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/80. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa. |
|
ICC/IF
1/50 - 1/100. |
Target
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Function
Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. -
Domain
The extracellular domain is homologous to the repeating units (of approximately 147 AA) of the cation-independent mannose 6-phosphate receptor. -
Cellular localization
Lysosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 4074 Human
- Entrez Gene: 17113 Mouse
- Entrez Gene: 100909548 Rat
- Entrez Gene: 312689 Rat
- Omim: 154540 Human
- SwissProt: P20645 Human
- SwissProt: P24668 Mouse
- SwissProt: Q6AY20 Rat
see all -
Alternative names
- 46 kDa mannose 6 phosphate receptor antibody
- 46 kDa mannose 6-phosphate receptor antibody
- Cation dependent mannose 6 phosphate receptor antibody
see all
Images
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Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Empty
Lane 3: M6PR knockout HAP1 whole cell lysate (20 µg)
Lane 4: A549 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - unpurified ab134153 observed at 46 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab134153 was shown to specifically react with M6PR when M6PR knockout samples were used. Wild-type and M6PR knockout samples were subjected to SDS-PAGE. ab134153 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)](https://www.abcam.com/ps/products/134/ab134153/Images/ab134153-314332-anti-m6pr-cation-dependent-antibody-epr7691-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling M6PR (cation dependent) with Purified ab134153 at 1:100 dilution (8.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor®488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)](https://www.abcam.com/ps/products/134/ab134153/Images/ab134153-314273-anti-m6pr-cation-dependent-antibody-epr7691-western-blot.jpg)
Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)All lanes : Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153) at 0.8 µg/ml (purified)
Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
Lane 2 : Mouse kidney lysates
Lane 3 : Rat kidney lysates
Lane 4 : Rat spleen lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 31 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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![Flow Cytometry (Intracellular) - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)](https://www.abcam.com/ps/products/134/ab134153/Images/ab134153-314339-anti-m6pr-cation-dependent-antibody-epr7691-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)Intracellular Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling M6PR (cation dependent) with purified ab134153 at 1/80 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)](https://www.abcam.com/ps/products/134/ab134153/Images/ab134153-148425-anti-m6pr-cation-dependent-antibody-epr7691-western-blot.jpg)
Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)All lanes : Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153) at 1/1000 dilution (Unpurified)
Lane 1 : A549 lysates
Lane 2 : Human uterus lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 31 kDa -
![OI-RD Scanning - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)](https://www.abcam.com/ps/products/134/ab134153/Images/ab134153-148424-anti-m6pr-cation-dependent-antibody-epr7691.jpg)
OI-RD Scanning - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153) -
![Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)](https://www.abcam.com/ps/products/134/ab134153/Images/ab134153-7-benefits-of-recombinant-antibodies.png)
Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab134153 has been referenced in 8 publications.
- Dieterich IA et al. Acetyl-CoA flux from the cytosol to the ER regulates engagement and quality of the secretory pathway. Sci Rep 11:2013 (2021). PubMed: 33479349
- Miserey-Lenkei S et al. A comprehensive library of fluorescent constructs of SARS-CoV-2 proteins and their initial characterisation in different cell types. Biol Cell 113:311-328 (2021). PubMed: 33666950
- Cui L et al. The Lysosomal Membrane Protein Lamp2 Alleviates Lysosomal Cell Death by Promoting Autophagic Flux in Ischemic Cardiomyocytes. Front Cell Dev Biol 8:31 (2020). PubMed: 32117965
- Fernández de Castro I et al. A modified lysosomal organelle mediates nonlytic egress of reovirus. J Cell Biol 219:N/A (2020). PubMed: 32356864
- Han J et al. Involvement of CASP9 (caspase 9) in IGF2R/CI-MPR endosomal transport. Autophagy N/A:1-17 (2020). PubMed: 32397873
- Han J et al. HSP90 inhibition targets autophagy and induces a CASP9-dependent resistance mechanism in NSCLC. Autophagy 14:958-971 (2018). PubMed: 29561705
- Siupka P et al. Bidirectional apical-basal traffic of the cation-independent mannose-6-phosphate receptor in brain endothelial cells. J Cereb Blood Flow Metab 37:2598-2613 (2017). PubMed: 28337939
- Di Stefano AL et al. Detection, Characterization, and Inhibition of FGFR-TACC Fusions in IDH Wild-type Glioma. Clin Cancer Res 21:3307-17 (2015). PubMed: 25609060