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  1. Link

    m6pr-cation-dependent-antibody-epr7691-ab134153.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (8)

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Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
  • Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
  • Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
  • Flow Cytometry (Intracellular) - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
  • Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
  • OI-RD Scanning - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
  • Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7691] to M6PR (cation dependent)
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Primary
Product image
Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Primary
Product image
HRP Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Loading Control (ab194109)

View more associated products

Overview

  • Product name

    Anti-M6PR (cation dependent) antibody [EPR7691]
    See all M6PR (cation dependent) primary antibodies
  • Description

    Rabbit monoclonal [EPR7691] to M6PR (cation dependent)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human M6PR (cation dependent) aa 250 to the C-terminus (C terminal).
    Database link: P20645

  • Positive control

    • WB: HAP1, A549, and Human uterus lysates ICC/IF: HeLa cell lysate Flow Cyt (intra): A549 cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 6.30 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7691
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Lysosome
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-M6PR (cation dependent) antibody [EPR7691] - BSA and Azide free (ab236067)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human M6PR (cation dependent) protein (ab80157)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/80.
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
ICC/IF (1)
1/50 - 1/100.
Notes
Flow Cyt (Intra)
1/80.
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
ICC/IF
1/50 - 1/100.
Application notes
Is unsuitable for IHC-P.

Target

  • Function

    Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex.
  • Domain

    The extracellular domain is homologous to the repeating units (of approximately 147 AA) of the cation-independent mannose 6-phosphate receptor.
  • Cellular localization

    Lysosome membrane.
  • Target information above from: UniProt accession P20645 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4074 Human
    • Entrez Gene: 17113 Mouse
    • Entrez Gene: 100909548 Rat
    • Entrez Gene: 312689 Rat
    • Omim: 154540 Human
    • SwissProt: P20645 Human
    • SwissProt: P24668 Mouse
    • SwissProt: Q6AY20 Rat
    • Unigene: 134084 Human
    • Unigene: 744902 Human
    • Unigene: 249225 Mouse
    • Unigene: 91427 Rat
    see all
  • Alternative names

    • 46 kDa mannose 6 phosphate receptor antibody
    • 46 kDa mannose 6-phosphate receptor antibody
    • Cation dependent mannose 6 phosphate receptor antibody
    • Cation dependent mannose 6 phosphate receptor precursor antibody
    • Cation-dependent mannose-6-phosphate receptor antibody
    • CD Man 6 P receptor antibody
    • CD Man-6-P receptor antibody
    • CD MPR antibody
    • CD-M6PR antibody
    • CD-MPR antibody
    • FLJ32994 antibody
    • M6pr antibody
    • M6PR protein antibody
    • Man6PR antibody
    • Mannose 6 phosphate receptor (cation dependent) antibody
    • Mannose 6 phosphate receptor antibody
    • MPR 46 antibody
    • MPR46 antibody
    • MPRD antibody
    • MPRD_HUMAN antibody
    • Mr 46,000 Man6PR antibody
    • Small mannose 6 phosphate receptor antibody
    • SMPR antibody
    see all

Images

  • Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
    Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: Empty
    Lane 3: M6PR knockout HAP1 whole cell lysate (20 µg)
    Lane 4: A549 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - unpurified ab134153 observed at 46 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab134153 was shown to specifically react with M6PR when M6PR knockout samples were used. Wild-type and M6PR knockout samples were subjected to SDS-PAGE. ab134153 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
    Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling M6PR (cation dependent) with Purified ab134153 at 1:100 dilution (8.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor®488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
    Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
    All lanes : Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153) at 0.8 µg/ml (purified)

    Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
    Lane 2 : Mouse kidney lysates
    Lane 3 : Rat kidney lysates
    Lane 4 : Rat spleen lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 31 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Flow Cytometry (Intracellular) - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
    Flow Cytometry (Intracellular) - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)

    Intracellular Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling M6PR (cation dependent) with purified ab134153 at 1/80 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

     

  • Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
    Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
    All lanes : Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153) at 1/1000 dilution (Unpurified)

    Lane 1 : A549 lysates
    Lane 2 : Human uterus lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 31 kDa

  • OI-RD Scanning - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
    OI-RD Scanning - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
    Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (8)

Publishing research using ab134153? Please let us know so that we can cite the reference in this datasheet.

ab134153 has been referenced in 8 publications.

  • Dieterich IA  et al. Acetyl-CoA flux from the cytosol to the ER regulates engagement and quality of the secretory pathway. Sci Rep 11:2013 (2021). PubMed: 33479349
  • Miserey-Lenkei S  et al. A comprehensive library of fluorescent constructs of SARS-CoV-2 proteins and their initial characterisation in different cell types. Biol Cell 113:311-328 (2021). PubMed: 33666950
  • Cui L  et al. The Lysosomal Membrane Protein Lamp2 Alleviates Lysosomal Cell Death by Promoting Autophagic Flux in Ischemic Cardiomyocytes. Front Cell Dev Biol 8:31 (2020). PubMed: 32117965
  • Fernández de Castro I  et al. A modified lysosomal organelle mediates nonlytic egress of reovirus. J Cell Biol 219:N/A (2020). PubMed: 32356864
  • Han J  et al. Involvement of CASP9 (caspase 9) in IGF2R/CI-MPR endosomal transport. Autophagy N/A:1-17 (2020). PubMed: 32397873
  • Han J  et al. HSP90 inhibition targets autophagy and induces a CASP9-dependent resistance mechanism in NSCLC. Autophagy 14:958-971 (2018). PubMed: 29561705
  • Siupka P  et al. Bidirectional apical-basal traffic of the cation-independent mannose-6-phosphate receptor in brain endothelial cells. J Cereb Blood Flow Metab 37:2598-2613 (2017). PubMed: 28337939
  • Di Stefano AL  et al. Detection, Characterization, and Inhibition of FGFR-TACC Fusions in IDH Wild-type Glioma. Clin Cancer Res 21:3307-17 (2015). PubMed: 25609060

Customer reviews and Q&As

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Question

Literature shows that band of glycosylated protein should be around 45 kDa, not 31 kDa.

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Abcam community

Verified customer

Asked on Jun 28 2012

Answer

Thank you for your call today and for letting us know about the trouble with ab76276.

As we discussed, I'm sending a free of charge vial of ab134153 on the order ***, which I expect to arrive by July 9. Please keep me updated about the results with this antibody.

I look forward to hearing from you. If there is anything else that I can dofor you in the meantime, please let me know and I'll be happy to help.

Have a nice day.

Read More

Abcam Scientific Support

Answered on Jun 28 2012

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