Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-M6PR (cation dependent) antibody [EPR7691] - BSA and Azide free (ab236067)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7691] to M6PR (cation dependent) - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-M6PR (cation dependent) antibody [EPR7691] - BSA and Azide free
    See all M6PR (cation dependent) primary antibodies

  • Description

    Rabbit monoclonal [EPR7691] to M6PR (cation dependent) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human M6PR (cation dependent) aa 250-350 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HAP1 and A549 cell lysates.

  • General notes

    Ab236067 is the carrier-free version of ab134153. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab236067 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab236067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.

Target

  • Function

    Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex.

  • Domain

    The extracellular domain is homologous to the repeating units (of approximately 147 AA) of the cation-independent mannose 6-phosphate receptor.

  • Cellular localization

    Lysosome membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 46 kDa mannose 6 phosphate receptor antibody
    • 46 kDa mannose 6-phosphate receptor antibody
    • Cation dependent mannose 6 phosphate receptor antibody
    • Cation dependent mannose 6 phosphate receptor precursor antibody
    • Cation-dependent mannose-6-phosphate receptor antibody
    • CD Man 6 P receptor antibody
    • CD Man-6-P receptor antibody
    • CD MPR antibody
    • CD-M6PR antibody
    • CD-MPR antibody
    • FLJ32994 antibody
    • M6pr antibody
    • M6PR protein antibody
    • Man6PR antibody
    • Mannose 6 phosphate receptor (cation dependent) antibody
    • Mannose 6 phosphate receptor antibody
    • MPR 46 antibody
    • MPR46 antibody
    • MPRD antibody
    • MPRD_HUMAN antibody
    • Mr 46,000 Man6PR antibody
    • Small mannose 6 phosphate receptor antibody
    • SMPR antibody
    see all

Images

  • Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] - BSA and Azide free (ab236067)
    Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] - BSA and Azide free (ab236067)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: Empty
    Lane 3: M6PR knockout HAP1 whole cell lysate (20 µg)
    Lane 4: A549 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab134153 observed at 46 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab134153 was shown to specifically react with M6PR when M6PR knockout samples were used. Wild-type and M6PR knockout samples were subjected to SDS-PAGE. ab134153 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134153).

  • Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation dependent) antibody [EPR7691] - BSA and Azide free (ab236067)
    Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation dependent) antibody [EPR7691] - BSA and Azide free (ab236067)
    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling M6PR (cation dependent) with Purified ab134153 at 1:100 dilution (8.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor®488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134153).

  • Flow Cytometry - Anti-M6PR (cation dependent) antibody [EPR7691] - BSA and Azide free (ab236067)
    Flow Cytometry - Anti-M6PR (cation dependent) antibody [EPR7691] - BSA and Azide free (ab236067)
    Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling M6PR (cation dependent) with purified ab134153 at 1:80 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134153).

  • OI-RD Scanning - Anti-M6PR (cation dependent) antibody [EPR7691] - BSA and Azide free (ab236067)
    OI-RD Scanning - Anti-M6PR (cation dependent) antibody [EPR7691] - BSA and Azide free (ab236067)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134153).

  • Anti-M6PR (cation dependent) antibody [EPR7691] - BSA and Azide free (ab236067)
    Anti-M6PR (cation dependent) antibody [EPR7691] - BSA and Azide free (ab236067)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab236067? Please let us know so that we can cite the reference in this datasheet.

ab236067 has not yet been referenced specifically in any publications.

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