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  1. Link

    m6pr-cation-independent-antibody-ab12894.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-M6PR (cation independent) antibody (ab12894)

  • Datasheet
  • SDS
Reviews (6)Q&A (2)References (5)

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Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody (ab12894)
  • Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody (ab12894)

Key features and details

  • Rabbit polyclonal to M6PR (cation independent)
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-M6PR (cation independent) antibody
    See all M6PR (cation independent) primary antibodies
  • Description

    Rabbit polyclonal to M6PR (cation independent)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Monkey, African green monkey
  • Immunogen

    Synthetic peptide corresponding to Human M6PR (cation independent) aa 700-800 (internal sequence) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab13210)

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Endosome
    • Signal Transduction
    • Protein Trafficking
    • Golgi Proteins
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human M6PR (cation independent) peptide (ab13210)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • Lysosomal Staining Kit - Blue Fluorescence - Cytopainter (ab112135)
    • Lysosomal Staining Kit - Red Fluorescence - Cytopainter (ab112137)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab12894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (3)
Use a concentration of 4 µg/ml. This antibody worked well in IF in HeLa cells but not in SK-N-SH cells.
Notes
ICC/IF
Use a concentration of 4 µg/ml. This antibody worked well in IF in HeLa cells but not in SK-N-SH cells.

Target

  • Function

    Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.
  • Sequence similarities

    Belongs to the MRL1/IGF2R family.
    Contains 1 fibronectin type-II domain.
  • Domain

    Contains 15 repeating units of approximately 147 AA harboring four disulfide bonds each. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends.
  • Cellular localization

    Lysosome membrane. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface.
  • Target information above from: UniProt accession P11717 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3482 Human
    • Entrez Gene: 16004 Mouse
    • Omim: 147280 Human
    • SwissProt: P11717 Human
    • SwissProt: Q07113 Mouse
    • Unigene: 487062 Human
    • Unigene: 673278 Human
    • Unigene: 26553 Mouse
    • Unigene: 486511 Mouse
    see all
  • Alternative names

    • 300 kDa mannose 6 phosphate receptor antibody
    • 300 kDa mannose 6-phosphate receptor antibody
    • Cation independent mannose 6 phosphate receptor antibody
    • Cation-independent mannose-6-phosphate receptor antibody
    • CD222 antibody
    • CD222 antigen antibody
    • CI Man 6 P receptor antibody
    • CI Man-6-P receptor antibody
    • CI MPR antibody
    • CI-M6PR antibody
    • CI-MPR antibody
    • CIMPR antibody
    • IGF 2 receptor antibody
    • IGF 2R antibody
    • IGF II receptor antibody
    • IGF-II receptor antibody
    • IGF2 receptor antibody
    • Igf2r antibody
    • Insulin like growth factor 2 receptor antibody
    • Insulin like growth factor II receptor antibody
    • Insulin-like growth factor 2 receptor antibody
    • Insulin-like growth factor II receptor antibody
    • M6P R antibody
    • M6P/IGF2 receptor antibody
    • M6P/IGF2R antibody
    • M6PR antibody
    • mannose 6 phosphate receptor antibody
    • mannose 6 phosphate receptor, cation independent antibody
    • MPR 300 antibody
    • MPR300 antibody
    • MPRI antibody
    • MPRI_HUMAN antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody (ab12894)
    Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody (ab12894)This image was submitted as part of a review by Kirk McManus, University of British Columbia

    HeLa cells labelled with anti-mannose-6-phosphate receptor (ab12894). ab12894 was used at a working concentration of 4µg/ml. The arrows indicate the perinuclear location of the mannose-6-phosphate receptor in the late endosomes. In the final panel DAPI is pseudo-coloured red, while the mannose-6-phosphate receptor is in green.

  • Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody (ab12894)
    Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody (ab12894)This image is courtesy of an anonymous Abreview
    ab12894 at 1/100 staining human MCF-7 cells by ICC/IF. The cells were methanol fixed and blocked with BSA prior to incubation with the antibody for 1 hour. A goat anti-rabbit FITC was used as the secondary.

    See Abreview

Protocols

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (5)

Publishing research using ab12894? Please let us know so that we can cite the reference in this datasheet.

ab12894 has been referenced in 5 publications.

  • Fong LK  et al. Full-length amyloid precursor protein regulates lipoprotein metabolism and amyloid-ß clearance in human astrocytes. J Biol Chem 293:11341-11357 (2018). PubMed: 29858247
  • Liu Y  et al. Endocytic processing of adeno-associated virus type 8 vectors for transduction of target cells. Gene Ther : (2012). Human . PubMed: 22622241
  • Shen Y  et al. Mutations in PNKD causing paroxysmal dyskinesia alters protein cleavage and stability. Hum Mol Genet 20:2322-32 (2011). ICC/IF ; Human . PubMed: 21487022
  • Nishitsuji K  et al. The E693Delta mutation in amyloid precursor protein increases intracellular accumulation of amyloid beta oligomers and causes endoplasmic reticulum stress-induced apoptosis in cultured cells. Am J Pathol 174:957-69 (2009). ICC/IF ; African green monkey . PubMed: 19164507
  • Clarke JH  et al. Distribution and neuronal expression of phosphatidylinositol phosphate kinase IIgamma in the mouse brain. J Comp Neurol 517:296-312 (2009). PubMed: 19757494

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Mannose 6 Phosphate Receptor (Cation independent) antibody - Late Endosome Marker

Poor
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HELA)
Specification
HELA
Fixative
Paraformaldehyde
Permeabilization
No
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 30 2012

Immunocytochemistry/ Immunofluorescence abreview for Anti-M6PR (cation independent) antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MCF-7)
Specification
MCF-7
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1%
Fixative
Methanol
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 01 2007

Immunocytochemistry/ Immunofluorescence abreview for Anti-M6PR (cation independent) antibody

Average
Abreviews
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa and SK-N-SH)
Specification
HeLa and SK-N-SH
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Kirk Mcmanus

Verified customer

Submitted Oct 12 2005

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