Anti-M6PR (cation independent) antibody (ab12894)
Key features and details
- Rabbit polyclonal to M6PR (cation independent)
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-M6PR (cation independent) antibody
See all M6PR (cation independent) primary antibodies -
Description
Rabbit polyclonal to M6PR (cation independent) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human, African green monkey
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to Human M6PR (cation independent) aa 700-800 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab13210) -
Positive control
- This antibody gave a positive signal in the following Methanol fixed cell lines: MCF-7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab12894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 4 µg/ml. This antibody worked well in IF in HeLa cells but not in SK-N-SH cells. |
Target
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Function
Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4. -
Sequence similarities
Belongs to the MRL1/IGF2R family.
Contains 1 fibronectin type-II domain. -
Domain
Contains 15 repeating units of approximately 147 AA harboring four disulfide bonds each. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends. -
Cellular localization
Lysosome membrane. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface. - Information by UniProt
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Database links
- Entrez Gene: 3482 Human
- Entrez Gene: 16004 Mouse
- Omim: 147280 Human
- SwissProt: P11717 Human
- SwissProt: Q07113 Mouse
- Unigene: 487062 Human
- Unigene: 673278 Human
- Unigene: 26553 Mouse
see all -
Alternative names
- 300 kDa mannose 6 phosphate receptor antibody
- 300 kDa mannose 6-phosphate receptor antibody
- Cation independent mannose 6 phosphate receptor antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody (ab12894)This image was submitted as part of a review by Kirk McManus, University of British ColumbiaHeLa cells labelled with anti-mannose-6-phosphate receptor (ab12894). ab12894 was used at a working concentration of 4µg/ml. The arrows indicate the perinuclear location of the mannose-6-phosphate receptor in the late endosomes. In the final panel DAPI is pseudo-coloured red, while the mannose-6-phosphate receptor is in green.
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Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody (ab12894)This image is courtesy of an anonymous Abreviewab12894 at 1/100 staining human MCF-7 cells by ICC/IF. The cells were methanol fixed and blocked with BSA prior to incubation with the antibody for 1 hour. A goat anti-rabbit FITC was used as the secondary.
References
ab12894 has been referenced in 5 publications.
- Fong LK et al. Full-length amyloid precursor protein regulates lipoprotein metabolism and amyloid-ß clearance in human astrocytes. J Biol Chem 293:11341-11357 (2018). PubMed: 29858247
- Liu Y et al. Endocytic processing of adeno-associated virus type 8 vectors for transduction of target cells. Gene Ther : (2012). Human . PubMed: 22622241
- Shen Y et al. Mutations in PNKD causing paroxysmal dyskinesia alters protein cleavage and stability. Hum Mol Genet 20:2322-32 (2011). ICC/IF ; Human . PubMed: 21487022
- Nishitsuji K et al. The E693Delta mutation in amyloid precursor protein increases intracellular accumulation of amyloid beta oligomers and causes endoplasmic reticulum stress-induced apoptosis in cultured cells. Am J Pathol 174:957-69 (2009). ICC/IF ; African green monkey . PubMed: 19164507
- Clarke JH et al. Distribution and neuronal expression of phosphatidylinositol phosphate kinase IIgamma in the mouse brain. J Comp Neurol 517:296-312 (2009). PubMed: 19757494
