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Staining of cells is diffuse in the cytoplasm and there is heavy background outside the cells.
Asked on Aug 13 2012
Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. The one protocol modification that might have an effect on the background stain would be to substitute either a different serum or BSA (1%) for the blocking donkey serum, but if you have not seen this issue with other antibodies in the same protocol with the same reagents, I am doubtful the susbstitution will help.
I have issued a free of charge replacement with the order number 1144831.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
Answered on Aug 13 2012