Key features and details
- Rabbit polyclonal to M6PR (cation independent) - C-terminal
- Suitable for: WB, IHC-P, ICC
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-M6PR (cation independent) antibody - C-terminal
See all M6PR (cation independent) primary antibodies
DescriptionRabbit polyclonal to M6PR (cation independent) - C-terminal
SpecificityNo cross reactivity with other proteins.
Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Cow
- Human intestinal cancer tissue, HCT116 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 274 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||Use a concentration of 0.5 - 1 µg/ml.|
FunctionTransport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.
Sequence similaritiesBelongs to the MRL1/IGF2R family.
Contains 1 fibronectin type-II domain.
DomainContains 15 repeating units of approximately 147 AA harboring four disulfide bonds each. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends.
Cellular localizationLysosome membrane. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface.
- Information by UniProt
- 300 kDa mannose 6 phosphate receptor antibody
- 300 kDa mannose 6-phosphate receptor antibody
- Cation independent mannose 6 phosphate receptor antibody
All lanes : Anti-M6PR (cation independent) antibody - C-terminal (ab191147) at 0.5 µg/ml
Lane 1 : HeLa Whole Cell Lysate
Lane 2 : Jurkat Whole Cell Lysate
Lane 3 : 22RV1 Whole Cell Lysate
Lane 4 : Hek293-T Whole Cell Lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 274 kDa
Observed band size: 274 kDa
Immunohistochemical analysis of paraffin-embedded Human intestinal cancer tissue cells labeling M6PR (cation independent) with ab191147 at 1 µg/ml.
Immunocytochemical analysis of HCT116 cells labeling M6PR (cation independent) with ab191147 at 1 µg/ml.
ab191147 has not yet been referenced specifically in any publications.