Western blot abreview for Anti-Mannose 6 Phosphate Receptor (Cation independent) antibody [EPR6599]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Hek293)
Loading amount
30 µg
Specification
Hek293
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Dilution
1/50000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: anti rabbit
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
HRP
Exposure
3 hour(s), 0 minute(s) and 30 second(s)
Bands
Specific: above 250 kDa

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Submitted Jan 29 2013

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