Product nameAnti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (Alexa Fluor® 405)
See all M6PR (cation independent) primary antibodies
DescriptionRabbit monoclonal [EPR6599] to M6PR (cation independent) - Lysosome Membrane Marker (Alexa Fluor® 405)
ConjugationAlexa Fluor® 405. Ex: 402nm, Em: 421nm
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide within Human M6PR (cation independent) aa 2450 to the C-terminus (C terminal). The exact sequence is proprietary.
(Peptide available as
- ICC/IF - CaCo2 cells
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or firstname.lastname@example.org.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Dissociation constant (KD)KD = 3.90 x 10 -12 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
- Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (ab124767)
- Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (Alexa Fluor® 488) (ab184807)
- Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (Alexa Fluor® 647) (ab198324)
- Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (Alexa Fluor® 568) (ab202535)
- Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (Alexa Fluor® 594) (ab203437)
- Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (Alexa Fluor® 555) (ab203438)
- Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (Allophycocyanin) (ab225131)
- Anti-M6PR (cation independent) antibody [EPR6599] (Phycoerythrin) (ab225132)
- Anti-M6PR (cation independent) antibody [EPR6599] - BSA and Azide free (ab226090)
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab198515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product gave a positive signal in CaCo2 cells fixed with 4% Formaldehyde (10 min) and 100% methanol (5 min).
FunctionTransport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.
Sequence similaritiesBelongs to the MRL1/IGF2R family.
Contains 1 fibronectin type-II domain.
DomainContains 15 repeating units of approximately 147 AA harboring four disulfide bonds each. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends.
Cellular localizationLysosome membrane. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface.
- Information by UniProt
- 300 kDa mannose 6 phosphate receptor antibody
- 300 kDa mannose 6-phosphate receptor antibody
- Cation independent mannose 6 phosphate receptor antibody
ab198515 staining M6PR in CaCo2 cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes, blocked with Image-iT® FX Signal Enhancer at room temperature for 30 min and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198515 at 1/100 dilution (shown in blue). Nuclear DNA was labelled with ab108410, DRAQ5™ at 1.25uM (1/4000 dilution) (shown in red).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in CaCo2 cells fixed with 100% methanol (5 min).
ab198515 has not yet been referenced specifically in any publications.