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Where is the epitope that the antibody recognizes located? Is it extracellular?
Asked on Oct 18 2011
Thank you for contacting us. The following two papers include protocols with this clone, MEM-238, which indicate that the antibody recognizes an extracellular epitope. This will also be the case for the FITC conjugate, ab58441. (The unconjugated version is catalogue ID ab8093). Leksa V et al. The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration. J Biol Chem 277:40575-82 (2002). WB, Flow Cyt; Human. PubMed: 12189157 Laube F Mannose-6-phosphate/insulin-like growth factor-II receptor in human melanoma cells: effect of ligands and antibodies on the receptor expression. Anticancer Res 29:1383-8 (2009). Inhib; Human. PubMed: 19414391 The flow cytometry protocol in the Leska paper used live cells, without permeabilization, and the inhibition assay in the Laube paper also used live cells. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Oct 18 2011