Anti-M6PR (cation independent) (phospho S2409) antibody (ab138453)
Key features and details
- Rabbit polyclonal to M6PR (cation independent) (phospho S2409)
- Suitable for: WB
- Reacts with: African green monkey
- Isotype: IgG
Overview
-
Product name
Anti-M6PR (cation independent) (phospho S2409) antibody
See all M6PR (cation independent) primary antibodies -
Description
Rabbit polyclonal to M6PR (cation independent) (phospho S2409) -
Host species
Rabbit -
Specificity
ab138453 detects endogenous levels of Mannose 6 Phosphate Receptor (Cation independent) only when phosphorylated at S2409. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: African green monkey
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Extracts from COS7 cells treated with UV for 15 minutes.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab138453 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab138453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/1000. Predicted molecular weight: 274 kDa.
|
Notes |
---|
WB
1/500 - 1/1000. Predicted molecular weight: 274 kDa. |
Target
-
Function
Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4. -
Sequence similarities
Belongs to the MRL1/IGF2R family.
Contains 1 fibronectin type-II domain. -
Domain
Contains 15 repeating units of approximately 147 AA harboring four disulfide bonds each. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends. -
Cellular localization
Lysosome membrane. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface. - Information by UniProt
-
Database links
- Entrez Gene: 16004 Mouse
- SwissProt: Q07113 Mouse
- Unigene: 26553 Mouse
- Unigene: 486511 Mouse
-
Alternative names
- 300 kDa mannose 6 phosphate receptor antibody
- 300 kDa mannose 6-phosphate receptor antibody
- Cation independent mannose 6 phosphate receptor antibody
see all
Images
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab138453 has been referenced in 2 publications.
- Allodi I et al. Differential neuronal vulnerability identifies IGF-2 as a protective factor in ALS. Sci Rep 6:25960 (2016). PubMed: 27180807
- Ting WJ et al. Environmental tobacco smoke increases autophagic effects but decreases longevity associated with Sirt-1 protein expression in young C57BL mice hearts. Oncotarget 7:39017-39025 (2016). WB . PubMed: 27167200