Key features and details
- Rabbit polyclonal to M6PR (cation independent) (phospho S2409)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-M6PR (cation independent) (phospho S2409) antibody
See all M6PR (cation independent) primary antibodies
DescriptionRabbit polyclonal to M6PR (cation independent) (phospho S2409)
Specificityab138453 detects endogenous levels of Mannose 6 Phosphate Receptor (Cation independent) only when phosphorylated at S2409.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic phospho-peptide against the C-terminal region surrounding S2409 of the Human Mannose 6 Phosphate Receptor (Cation independent) protein.
- WB: Extracts from COS7 cells treated with UV for 15 minutes.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab138453 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Our Abpromise guarantee covers the use of ab138453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 274 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionTransport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.
Sequence similaritiesBelongs to the MRL1/IGF2R family.
Contains 1 fibronectin type-II domain.
DomainContains 15 repeating units of approximately 147 AA harboring four disulfide bonds each. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends.
Cellular localizationLysosome membrane. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface.
- Information by UniProt
- 300 kDa mannose 6 phosphate receptor antibody
- 300 kDa mannose 6-phosphate receptor antibody
- Cation independent mannose 6 phosphate receptor antibody
ab138453 has been referenced in 2 publications.
- Allodi I et al. Differential neuronal vulnerability identifies IGF-2 as a protective factor in ALS. Sci Rep 6:25960 (2016). PubMed: 27180807
- Ting WJ et al. Environmental tobacco smoke increases autophagic effects but decreases longevity associated with Sirt-1 protein expression in young C57BL mice hearts. Oncotarget 7:39017-39025 (2016). WB . PubMed: 27167200