Anti-mH2A2 antibody [CL5263] (ab242357)
Key features and details
- Mouse monoclonal [CL5263] to mH2A2
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-mH2A2 antibody [CL5263]
See all mH2A2 primary antibodies -
Description
Mouse monoclonal [CL5263] to mH2A2 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Human mH2A2 aa 100-200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9P0M6 -
Positive control
- IHC-P: Human testis, fallopian tube, cerebral cortex and kidney tissue. ICC/IF: PC-3 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL5263 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab242357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 2 - 10 µg/ml.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 2 - 10 µg/ml. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation. -
Sequence similarities
Contains 1 histone H2A domain.
Contains 1 Macro domain. -
Cellular localization
Nucleus. Chromosome. Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin. - Information by UniProt
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Database links
- Entrez Gene: 55506 Human
- Entrez Gene: 404634 Mouse
- SwissProt: Q9P0M6 Human
- SwissProt: Q8CCK0 Mouse
- Unigene: 499953 Human
- Unigene: 272870 Mouse
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Alternative names
- Core histone macro H2A2.2 antibody
- Core histone macro-H2A.2 antibody
- Core histone macroH2A2.2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence anaysis of PC-3 cells labeling mH2A2 with ab242357 at 2-10 µg/mL. Cells were fixed with PFA and permeabilised with Triton X-100. The data is showing specific staining in the nucleoplasm and nuclear membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
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Paraffin-embedded human testis tissue stained for mH2A2 with ab242357 at 1/200 dilution in immunohistochemical analysis.
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Paraffin-embedded human fallopian tube tissue stained for mH2A2 with ab242357 at 1/200 dilution in immunohistochemical analysis.
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Paraffin-embedded human cerebral cortex tissue stained for mH2A2 with ab242357 at 1/200 dilution in immunohistochemical analysis.
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Paraffin-embedded human kidney tissue stained for mH2A2 with ab242357 at 1/200 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab242357 has not yet been referenced specifically in any publications.