• Product name
  • Description
    Rabbit polyclonal to MACC1
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human MACC1 (internal sequence). MACC1 antibody was raised against a 17 amino acid synthetic peptide near the center of human MACC1
    Database link: Q6ZN28

  • Positive control
    • Mouse liver tissue lysate; Human liver tissue IF: A431 cell line



Our Abpromise guarantee covers the use of ab106579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 97 kDa.
IHC-P Use a concentration of 2.5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.


  • Function
    Acts as a transcription activator for MET and as a key regulator of HGF-MET signaling. Promotes cell motility, proliferation and hepatocyte growth factor (HGF)-dependent scattering in vitro and tumor growth and metastasis in vivo.
  • Tissue specificity
    Preferentially expressed in metastasizing tumors.
  • Sequence similarities
    Contains 1 SH3 domain.
  • Cellular localization
    Cytoplasm. Nucleus. Mainly found in the cytoplasm in non-metastasizing tumors.
  • Information by UniProt
  • Database links
  • Alternative names
    • 7A5 antibody
    • MACC1 antibody
    • MACC1_HUMAN antibody
    • Metastasis associated in colon cancer 1 antibody
    • Metastasis-associated in colon cancer protein 1 antibody
    • OTTHUMP00000201891 antibody
    • Putative binding protein 7a5 antibody
    • SH3 domain-containing protein 7a5 antibody
    • SH3BP4L antibody
    see all


  • Western blot analysis of MACC1 expression in human liver tissue lysate with ab106579 at (A) 1 and (B) 2µg/ml.
  • Lane 1 : Anti-MACC1 antibody (ab106579) at 1 µg/ml
    Lane 2 : Anti-MACC1 antibody (ab106579) at 2 µg/ml

    All lanes : Mouse liver tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 97 kDa

  • Immunohistochemical analysis of MACC1 expression in paraffin embedded Human liver tissue, using ab106579 at 2.5 µg/ml.
  • ICC/IF image of ab106579 stained A431 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab106579, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Lane 1 : Anti-MACC1 antibody (ab106579) at 1 µg/ml
    Lane 2 : Anti-MACC1 antibody (ab106579) at 2 µg/ml

    All lanes : Human liver tissue lysate

    Predicted band size: 97 kDa

  • Immunofluorescence of MACC1 in Human Liver cells using ab106579 at 20 ug/ml.


This product has been referenced in:
  • Ma L  et al. Long non-coding RNA XIST promotes cell growth and invasion through regulating miR-497/MACC1 axis in gastric cancer. Oncotarget 8:4125-4135 (2017). WB ; Human . Read more (PubMed: 27911852) »
  • Zhou W  et al. Combination of Endothelial-Monocyte-Activating Polypeptide-II with Temozolomide Suppress Malignant Biological Behaviors of Human Glioblastoma Stem Cells via miR-590-3p/MACC1 Inhibiting PI3K/AKT/mTOR Signal Pathway. Front Mol Neurosci 10:68 (2017). Read more (PubMed: 28348518) »

See all 5 Publications for this product

Customer reviews and Q&As

Thank you for contacting us. Your credit note ID is 18744. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice ...

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