Overview

  • Product name

  • Description

    Rabbit polyclonal to MACF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse MACF1 aa 3651-3846.
    Sequence:

    KLSPQELT ALREKLHQAK EQYEVLQERT RVAQKELEEA VTSALQQETE KSKAATELAE NKRKIDALLD WVASVGSSER KPQASLPGME QFSGACLEKQ TLAATDGHVD VNQVPETLDR QYELMKARHQ ELLSQQQNFI VATQSVQSFL DQHSHNLTPE ERQKLQEKLG ELKEQYAASL ARSEAELKQT QALRDEL


    Database link: Q9QXZ0

  • Positive control

    • Mouse kidney tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Constituent: Proprietary component
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab117418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
WB 1/500. Predicted molecular weight: 832 kDa.
ELISA 1/10000.

Target

  • Relevance

    Microtubule-actin crosslinking factor 1 (MACF1/ACF7) belongs to spectraplakin family of proteins which also includes mammalian Bpag1/dystonin, Drosophila Short Stop (Shot) and Caenorhabditis elegans Vab-10. These are large actin-microtubule linker molecules that are essential for various biological processes such as neuronal degeneration, wound healing, gastrulation. MACF1, located on mouse chromosome 4D2.2, was found to play an important role during neurogenesis- it was shown that MACF1 regulates the organization of neuronal microtubules which is essential for axon extension. Furthermore, MACF1 is required in controlling focal adhesion assembly and dynamics. MACF1 behaves as an actin-regulated ATPase and it has the ability to target microtubules that track along F-actin to focal adhesions. MACF1 was also implicated in regulating filopodia formation.
  • Cellular localization

    Cytoplasmic, microtubule. Plasma membrane. Golgi apparatus.
  • Database links

  • Alternative names

    • ABP620 antibody
    • Acf7 antibody
    • Aclp7 antibody
    • Actin cross linking family 7 antibody
    • MACF antibody
    • Microtubule actin crosslinking factor 1 antibody
    • mKIAA0465 antibody
    • R74989 antibody
    • rabeculin alpha antibody
    see all

Images

  • ab117418 at 1/500 dilution staining MACF1 in Mouse kidney tissue by Immunofluorescence.

References

ab117418 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for sending the protocol again. This time it arrived with the message.

The first thing I recommend checking is whether the transfer worked. You can do this with a Ponceau Red stain, before you incubate with the antibody. The protein is so big (832 kDa for the precursor) that transfer may not be efficient. Can you please tell me what your transfer buffer contains? Please let me know if you need a Ponceau Red staining protocol.

Can you also tell what kind cells or tissue you are loading into the gel? Are you also loading purified protein? If so, what is the molecular weight? How much of each sample do you load into the gel?

I see that the secondary is anti-mouse IgG, but ab117418 is a rabbit antibody. Have you tried an anti-rabbit secondary?

I look forward to your reply.

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