Key features and details
- Rat monoclonal [BM8] to Macrophage (PE)
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Product nameAnti-Macrophage antibody [BM8] (PE)
See all Macrophage primary antibodies
DescriptionRat monoclonal [BM8] to Macrophage (PE)
ConjugationPE. Ex: 488nm, Em: 575nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
BALB/c macrophages obtained from 14-day-old bone marrow cell cultures.
- Flow Cyt: C57Bl/6 bone marrow cells.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.16% Sodium phosphate, 0.1% Gelatin
Concentration information loading...
Our Abpromise guarantee covers the use of ab125148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
RelevanceMacrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becomming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognised as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).
- macrophages antibody
C57Bl/6 bone marrow cells were stained with APC Anti-Mouse CD11b (ab25482) and 0.25µg PE Anti-Mouse F4/80 Antigen (ab125148) (right panel) or 0.25µg PE Rat IgG2b isotype control (left panel).
ab125148 has been referenced in 1 publication.
- Leenen PJ et al. Markers of mouse macrophage development detected by monoclonal antibodies. J Immunol Methods 174:5-19 (1994). PubMed: 8083537