Key features and details
- Mouse monoclonal [PM-2K] to Macrophage
- Suitable for: WB, IHC-Fr
- Reacts with: Cow, Cat, Dog, Human, Pig, Monkey
- Isotype: IgG1
Product nameAnti-Macrophage antibody [PM-2K]
See all Macrophage primary antibodies
DescriptionMouse monoclonal [PM-2K] to Macrophage
SpecificityDoes not recognize Macrophage Scavenger Receptor AI or AII as determined using positive CHO cells.
Tested applicationsSuitable for: WB, IHC-Frmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Cow, Cat, Dog, Human, Pig, Monkey
Does not react with: Rat, Rabbit, Goat, Horse, Guinea pig, Hamster
Cultured human peritoneal macrophages.
- Macrophage cell membranes.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab58822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|IHC-Fr||1/200 - 1/800.|
RelevanceMacrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becomming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognised as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).
- macrophages antibody
ab58822 has been referenced in 2 publications.
- Marshall DC et al. Selective Allosteric Inhibition of MMP9 Is Efficacious in Preclinical Models of Ulcerative Colitis and Colorectal Cancer. PLoS One 10:e0127063 (2015). IHC . PubMed: 25961845
- Tsai YM et al. Functional interaction of cockroach allergens and mannose receptor (CD206) in human circulating fibrocytes. PLoS One 8:e64105 (2013). Flow Cyt ; Human . PubMed: 23734186