Anti-Macrophage antibody [RM0029-11H3] (ab56297)


  • Product name
    Anti-Macrophage antibody [RM0029-11H3]
    See all Macrophage primary antibodies
  • Description
    Rat monoclonal [RM0029-11H3] to Macrophage
  • Host species
  • Tested applications
    Suitable for: ICC/IF, IP, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Isolated mouse peritoneal macrophages

  • Positive control
    • Spleen, Lymph node, Diseased (GBM) mouse kidney tissue. IF/ICC: RAW246.7



Our Abpromise guarantee covers the use of ab56297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
IP 1/100.
Flow Cyt Use 2µg for 106 cells.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


IHC-P Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.


  • Relevance
    Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becomming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognised as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).
  • Alternative names
    • macrophages antibody


  • ICC/IF image of ab56297 stained RAW246.7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56297, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96887, DyLight® 488 goat anti-rat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • Bouin’s solution fixed and paraffin embedded mouse kidney section from anti-GBM model was subjected to immunohistochemistry staining (ABC) of Macrophage using ab56297.

    Bouin’s solution fixed and paraffin embedded mouse kidney section from anti-GBM model was subjected to immunohistochemistry staining (ABC) of Macrophage using ab56297.
  • Ab56297 staining macrophage in mouse brain tumour tissue sections by (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 0.5% TNB blocking reagent for 30 minutes at 25°C. Samples were incubated with primary antibody at 1/100 dilution for 18 hours at 4 C. A goat anti-rat IgG H&L (HRP) (ab7097) was used at 1/500 dilution.

    See Abreview

  • Overlay histogram showing RAW 264.7 cells stained with ab56297 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56297, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a, kappa monoclonal [aRTK2758] (ab18450, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in RAW 264.7 cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Immunohistochemical analysis of murine tumour tissue, staining Macrophage with ab56297. Antigen retrieval was performed under high pressure in 10 mM EDTA buffer (pH 8.0) before incubation with primary antibody.


This product has been referenced in:
  • Baudoux T  et al. CD4+ and CD8+ T Cells Exert Regulatory Properties During Experimental Acute Aristolochic Acid Nephropathy. Sci Rep 8:5334 (2018). IHC-P ; Mouse . Read more (PubMed: 29593222) »
  • Elshaer SL  et al. Deletion of TXNIP Mitigates High-Fat Diet-Impaired Angiogenesis and Prevents Inflammation in a Mouse Model of Critical Limb Ischemia. Antioxidants (Basel) 6:N/A (2017). Read more (PubMed: 28661427) »

See all 17 Publications for this product

Customer reviews and Q&As

Thank you for your reply.

I will contact * and determine the best way to get the FOCR sent to you as quickly as possible. Once we have sorted the situation out, we will get back to you and let you know when you should have the free of charge r...

Read More

Thank you for your reply.

I would be happy to credit the cost of the antibody back to you, or I could send one of the antibodies that the customer would like to try as a free of charge replacement instead.

Please let me know how you w...

Read More

Thank you for contacting Abcam.

The antibody is covered under our Abpromise for six months and is guaranteed to work in IHC-P on mouse samples . If we cannot resolve the issue you are having with the antibody then I would be happy to either s...

Read More

Thank you for contacting us. We have not tested this product to see whether it cross-reacts with monocytes.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.


Read More

We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field and do not want to recommend a product that may be unsuitable. We encourage customers to consult the lat...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (brain tumor)
brain tumor
Antigen retrieval step
Blocking step
(agent) for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 22 2009


Sign up