Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody (ab9781)


  • Product name

    Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody
    See all Macrophage Inflammatory Protein 1 alpha / CCL3 primary antibodies
  • Description

    Rabbit polyclonal to Macrophage Inflammatory Protein 1 alpha / CCL3
  • Host species

  • Tested applications

    Suitable for: Indirect ELISA, WB, ELISA, Neutralising, IHC-Frmore details
  • Species reactivity

    Reacts with: Rat
  • Immunogen

    Highly pure (>98%) recombinant rMIP-1-alpha (Rat macrophage inflammatory protein-1 alpha)

  • Positive control

    • Recombinant rat Macrophage Inflammatory Protein 1 alpha / CCL3 (ab9782) can be used as a positive control in WB.



Our Abpromise guarantee covers the use of ab9781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA
  • Application notes
    Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of rMIP-1a (100 ng/ml), a concentration of 8.0 - 10.0 µg/ml of this antibody is required.

    ELISA:To detect rMIP-1-alpha by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rMIP-1-alpha.

    Western Blot:To detect rMIP-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rMIP-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
  • Target

    • Function

      Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
    • Sequence similarities

      Belongs to the intercrine beta (chemokine CC) family.
    • Post-translational

      N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • C C motif chemokine 3 antibody
      • CCL 3 antibody
      • CCL3 antibody
      • CCL3_HUMAN antibody
      • Chemokine (C C motif) ligand 3 antibody
      • Chemokine C C motif ligand 3 antibody
      • Chemokine ligand 3 antibody
      • G0/G1 switch regulatory protein 19 1 antibody
      • G0/G1 switch regulatory protein 19-1 antibody
      • G0S19 1 antibody
      • G0S19 1 protein antibody
      • Heparin binding chemotaxis protein antibody
      • L2G25B antibody
      • LD78 alpha antibody
      • LD78-alpha(4-69) antibody
      • LD78alpha antibody
      • Macrophage inflammatory protein 1 alpha antibody
      • Macrophage inflammatory protein 1-alpha antibody
      • macrophage inflammatory protein 1a antibody
      • MIP 1 alpha antibody
      • MIP 1A antibody
      • MIP-1-alpha antibody
      • MIP-1-alpha(4-69) antibody
      • MIP1 alpha antibody
      • MIP1A antibody
      • PAT 464.1 antibody
      • SCYA 3 antibody
      • SCYA3 antibody
      • SIS alpha antibody
      • SIS beta antibody
      • SIS-beta antibody
      • Small inducible cytokine A3 antibody
      • small inducible cytokine A3 (homologous to mouse Mip-1a) antibody
      • Small-inducible cytokine A3 antibody
      • Tonsillar lymphocyte LD78 alpha protein antibody
      see all


    • Indirect ELISA measuring Macrophage Inflammatory Protein 1 alpha / CCL3 levels using ab9781.

      Supernatants collected from rat cerebral cortical cells after various treatments were coated onto 96-well flat bottom plates and incubated overnight at 4°C. The plates were blocked with 1% BSA at 37°C for 45 min before incubation with primary antibody (1/1000) in bicarbonate buffer overnight at 4°C. An HRP-conjugated secondary IgG (1/1000) was added and incubated for 45 min at 37°C, in the dark. The reaction was developed by adding o-phenylene diamine H2O2 for 20 min. Optical density was measured at 450 nm.


    This product has been referenced in:

    • Xue N  et al. Plasma Macrophage Migration Inhibitory Factor and CCL3 as Potential Biomarkers for Distinguishing Patients with Nasopharyngeal Carcinoma from High-Risk Individuals Who Have Positive Epstein-Barr Virus Capsid Antigen-Specific IgA. Cancer Res Treat 51:378-390 (2019). Read more (PubMed: 29807404) »
    • Johnson EA  et al. Increased expression of the chemokines CXCL1 and MIP-1a by resident brain cells precedes neutrophil infiltration in the brain following prolonged soman-induced status epilepticus in rats. J Neuroinflammation 8:41 (2011). IHC-Fr ; Rat . Read more (PubMed: 21535896) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Western blot
    Rat Tissue lysate - whole (liver)
    Loading amount
    30 µg
    LPS 1 hour
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Dr. Haoshu Fang

    Verified customer

    Submitted Nov 19 2012


    Thank you for contacting Abcam.

    All of the antibodies that you mentioned are guaranteed under our Abpromise to work in a Neutralizing assay, in various species:

    ab89546 - Works human samples
    ab9673 – Works in human samples
    ab9927- Works in mouse samples
    ab10381- Works in mouse and human
    ab10382 -Works in mouse samples

    If you are interested in larger quantities, then either just let me know which of the above antibodies you are interested in and I will pass your information onto our in-house sales team that specialize in bulk sales and they will be able to get you the best pricing possible.

    If there is anything else I can help you with, please let me know.

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