Product nameAnti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody
See all Macrophage Inflammatory Protein 1 alpha / CCL3 primary antibodies
DescriptionRabbit polyclonal to Macrophage Inflammatory Protein 1 alpha / CCL3
Tested applicationsSuitable for: Indirect ELISA, WB, ELISA, Neutralising, IHC-Frmore details
Species reactivityReacts with: Rat
Highly pure (>98%) recombinant rMIP-1-alpha (Rat macrophage inflammatory protein-1 alpha)
- Recombinant rat Macrophage Inflammatory Protein 1 alpha / CCL3 (ab9782) can be used as a positive control in WB.
FormLyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPBS, pH 7.4, no preservative, sterile filtered
Concentration information loading...
PurityImmunogen affinity purified
Light chain typeunknown
Our Abpromise guarantee covers the use of ab9781 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA:To detect rMIP-1-alpha by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rMIP-1-alpha.
Western Blot:To detect rMIP-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rMIP-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
FunctionMonokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
modificationsN-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.
- Information by UniProt
- C C motif chemokine 3 antibody
- CCL 3 antibody
- CCL3 antibody
Indirect ELISA measuring Macrophage Inflammatory Protein 1 alpha / CCL3 levels using ab9781.
Supernatants collected from rat cerebral cortical cells after various treatments were coated onto 96-well flat bottom plates and incubated overnight at 4°C. The plates were blocked with 1% BSA at 37°C for 45 min before incubation with primary antibody (1/1000) in bicarbonate buffer overnight at 4°C. An HRP-conjugated secondary IgG (1/1000) was added and incubated for 45 min at 37°C, in the dark. The reaction was developed by adding o-phenylene diamine H2O2 for 20 min. Optical density was measured at 450 nm.
This product has been referenced in:
- Johnson EA et al. Increased expression of the chemokines CXCL1 and MIP-1a by resident brain cells precedes neutrophil infiltration in the brain following prolonged soman-induced status epilepticus in rats. J Neuroinflammation 8:41 (2011). IHC-Fr ; Rat . Read more (PubMed: 21535896) »
- Tripathy D & Grammas P Acetaminophen protects brain endothelial cells against oxidative stress. Microvasc Res 77:289-96 (2009). ELISA ; Rat . Read more (PubMed: 19265712) »