Recombinant
RabMAb

Recombinant Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR16618-90] - BSA and Azide free (ab229699)

Overview

  • Product name

    Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR16618-90] - BSA and Azide free
    See all Macrophage Inflammatory Protein 1 alpha / CCL3 primary antibodies
  • Description

    Rabbit monoclonal [EPR16618-90] to Macrophage Inflammatory Protein 1 alpha / CCL3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IP, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant full length protein within Mouse Macrophage Inflammatory Protein 1 alpha/ CCL3 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P10855

  • Positive control

    • ICC/IF: RAW 264.7 cells treated with 100 ng/ml Lipopolysaccharide for 3h, followed by 300 ng/ml Brefeldin A for 3h.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®). 

    ab229699 is a PBS-only buffer format of ab179638. Please refer to ab179638 for recommended dilutions, protocols, and image data.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab229699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).

Target

  • Function

    Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications

    N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • C C motif chemokine 3 antibody
    • CCL 3 antibody
    • CCL3 antibody
    • CCL3_HUMAN antibody
    • Chemokine (C C motif) ligand 3 antibody
    • Chemokine C C motif ligand 3 antibody
    • Chemokine ligand 3 antibody
    • G0/G1 switch regulatory protein 19 1 antibody
    • G0/G1 switch regulatory protein 19-1 antibody
    • G0S19 1 antibody
    • G0S19 1 protein antibody
    • Heparin binding chemotaxis protein antibody
    • L2G25B antibody
    • LD78 alpha antibody
    • LD78-alpha(4-69) antibody
    • LD78alpha antibody
    • Macrophage inflammatory protein 1 alpha antibody
    • Macrophage inflammatory protein 1-alpha antibody
    • macrophage inflammatory protein 1a antibody
    • MIP 1 alpha antibody
    • MIP 1A antibody
    • MIP-1-alpha antibody
    • MIP-1-alpha(4-69) antibody
    • MIP1 alpha antibody
    • MIP1A antibody
    • PAT 464.1 antibody
    • SCYA 3 antibody
    • SCYA3 antibody
    • SIS alpha antibody
    • SIS beta antibody
    • SIS-beta antibody
    • Small inducible cytokine A3 antibody
    • small inducible cytokine A3 (homologous to mouse Mip-1a) antibody
    • Small-inducible cytokine A3 antibody
    • Tonsillar lymphocyte LD78 alpha protein antibody
    see all

Images

  • Macrophage Inflammatory Protein 1 alpha / CCL3 was immunoprecipitated from 0.35 mg RAW 264.7 (mouse  macrophage cell line transformed with Abelson murine leukemia virus), treated with 100 ng/ml Lipopolysaccharide for 3 hours, then add 300 ng/ml Brefeldin A for the last 3 hours, whole cell lysate with ab179638 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab179638 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: RAW 264.7 treated with 100 ng/ml Lipopolysaccharide for 3 hours, then add 300 ng/ml Brefeldin A for the last 3 hours whole cell lysate 10 µg (Input).

    Lane 2: ab179638 IP in RAW 264.7 treated with 100 ng/ml Lipopolysaccharide for 3 hours, then add 300 ng/ml Brefeldin A for the last 3 hours whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab179638 in RAW 264.7 treated with 100 ng/ml Lipopolysaccharide for 3 hours, then add 300 ng/ml Brefeldin A for the last 3 hours whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    High sensitivity ECL substrate used to develop the blot.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179638).

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized RAW 264.7 (mouse  macrophage cell line transformed with Abelson murine leukemia virus) cell line, treated with 100 ng/ml Lipopolysaccharide for 3 hours, then add 300 ng/ml Brefeldin A for the last 3 hours (red) / Untreated control (green), labeling Macrophage Inflammatory Protein 1 alpha / CCL3 with ab179638 at 1/600 compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179638).

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) untreated or treated with 100 ng/ml Lipopolysaccharide for 3 hours, followed by 300 ng/ml Brefeldin A for 3 hours cells labeling Macrophage Inflammatory Protein 1 alpha / CCL3 with ab179638 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing positive staining in RAW 264.7 treated with 100 ng/ml Lipopolysaccharide for 3 hours, followed by 300 ng/ml Brefeldin A for 3 hours.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179638).

     

References

ab229699 has not yet been referenced specifically in any publications.

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