Recombinant
RabMAb

Recombinant Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR19900-275] (ab206429)

Overview

  • Product name

    Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR19900-275]
    See all Macrophage Inflammatory Protein 1 alpha / CCL3 primary antibodies
  • Description

    Rabbit monoclonal [EPR19900-275] to Macrophage Inflammatory Protein 1 alpha / CCL3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Macrophage Inflammatory Protein 1 alpha/ CCL3 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P10147

  • Positive control

    • WB: THP-1 (treated with 80nM TPA overnight, then 100 ng/ml LPS for 3 hours and then 300 ng/ml Brefeldin A added for the last 3 hours) whole cell lysate. ICC/IF: THP-1 cells (treated with 80nM TPA overnight, then treated with 100ng/ml LPS for 3h and together with 300ng/ml BFA for another 3h). Flow cyt: THP-1 cells (treated with 80nM TPA overnight, then treated with 100ng/ml LPS for 3h and together with 300ng/ml BFA for another 3h).
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab206429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa).
ICC/IF 1/100.
Flow Cyt 1/600.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
    • Sequence similarities

      Belongs to the intercrine beta (chemokine CC) family.
    • Post-translational
      modifications

      N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • C C motif chemokine 3 antibody
      • CCL 3 antibody
      • CCL3 antibody
      • CCL3_HUMAN antibody
      • Chemokine (C C motif) ligand 3 antibody
      • Chemokine C C motif ligand 3 antibody
      • Chemokine ligand 3 antibody
      • G0/G1 switch regulatory protein 19 1 antibody
      • G0/G1 switch regulatory protein 19-1 antibody
      • G0S19 1 antibody
      • G0S19 1 protein antibody
      • Heparin binding chemotaxis protein antibody
      • L2G25B antibody
      • LD78 alpha antibody
      • LD78-alpha(4-69) antibody
      • LD78alpha antibody
      • Macrophage inflammatory protein 1 alpha antibody
      • Macrophage inflammatory protein 1-alpha antibody
      • macrophage inflammatory protein 1a antibody
      • MIP 1 alpha antibody
      • MIP 1A antibody
      • MIP-1-alpha antibody
      • MIP-1-alpha(4-69) antibody
      • MIP1 alpha antibody
      • MIP1A antibody
      • PAT 464.1 antibody
      • SCYA 3 antibody
      • SCYA3 antibody
      • SIS alpha antibody
      • SIS beta antibody
      • SIS-beta antibody
      • Small inducible cytokine A3 antibody
      • small inducible cytokine A3 (homologous to mouse Mip-1a) antibody
      • Small-inducible cytokine A3 antibody
      • Tonsillar lymphocyte LD78 alpha protein antibody
      see all

    Images

    • All lanes : Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR19900-275] (ab206429) at 1/1000 dilution

      Lane 1 : Untreated THP-1 (human monocytic leukemia cell line) whole cell lysate
      Lane 2 : THP-1 (1 treated with 80nM TPA overnight, then 100 ng/ml LPS for 3 hours and then 300 ng/ml Brefeldin A was added for the last 3 hours) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 10 kDa
      Observed band size: 12 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 (human monocytic leukemia cell line) cells labeling Macrophage Inflammatory Protein 1 alpha / CCL3 with ab206429 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in THP-1 cells treated with TPA (80nM overnight), then treated with 100ng/ml LPS for 3h and together with 300ng/ml BFA for another 3h. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Flow cytometric analysis of 4% paraformaldehyde-fixed, 0.1% Tween 20 permeabilized THP-1 (human monocytic leukemia cell line) (treated with 80nM TPA overnight, then treated with 100ng/ml LPS for 3h and together with 300ng/ml BFA for another 3 hr) (red) or untreated (green) cells labeling Macrophage Inflammatory Protein 1 alpha / CCL3 with ab206429 at 1/600 dilution compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluo 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

    References

    ab206429 has not yet been referenced specifically in any publications.

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