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  1. Link

    macrophage-inflammatory-protein-1-alpha--ccl3-antibody-epr22529-19-ab229900.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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RecombinantRabMAb

Recombinant Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR22529-19] (ab229900)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR22529-19] (ab229900)
  • Immunoprecipitation - Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR22529-19] (ab229900)
  • Flow Cytometry - Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR22529-19] (ab229900)
  • Immunocytochemistry/ Immunofluorescence - Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR22529-19] (ab229900)
  • Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR22529-19] (ab229900)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22529-19] to Macrophage Inflammatory Protein 1 alpha / CCL3
  • Suitable for: WB, Flow Cyt, ICC/IF, IP
  • Reacts with: Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Protein
Recombinant human Macrophage Inflammatory Protein 1 alpha / CCL3 (Animal Free) (ab217446)

View more associated products

Overview

  • Product name

    Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR22529-19]
    See all Macrophage Inflammatory Protein 1 alpha / CCL3 primary antibodies
  • Description

    Rabbit monoclonal [EPR22529-19] to Macrophage Inflammatory Protein 1 alpha / CCL3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Macrophage Inflammatory Protein 1 alpha/ CCL3 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P10147

  • Positive control

    • WB: THP-1 (treated with PMA and LPS) whole cell lysate. IP: THP-1 (treated with PMA and LPS) whole cell lysate. ICC/IF: THP-1 (treated with PMA and LPS) cells. Flow cyt: THP-1 (treated with PMA and LPS) cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22529-19
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Innate Immunity
    • Chemokines
    • Beta Chemokines (CC)
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Blood cell antigens ELISA kits
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • HIV-related

Associated products

  • Alternative Versions

    • Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR22529-19] - BSA and Azide free (ab254289)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human Macrophage Inflammatory Protein 1 alpha / CCL3 (Animal Free) (ab217446)
  • Related Products

    • Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

Applications

Our Abpromise guarantee covers the use of ab229900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 10 kDa.
Flow Cyt 1/500.
ICC/IF 1/100.
IP 1/30.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
    • Sequence similarities

      Belongs to the intercrine beta (chemokine CC) family.
    • Post-translational
      modifications

      N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P10147 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6348 Human
      • Omim: 182283 Human
      • SwissProt: P10147 Human
      • Unigene: 514107 Human
      • Alternative names

        • C C motif chemokine 3 antibody
        • CCL 3 antibody
        • CCL3 antibody
        • CCL3_HUMAN antibody
        • Chemokine (C C motif) ligand 3 antibody
        • Chemokine C C motif ligand 3 antibody
        • Chemokine ligand 3 antibody
        • G0/G1 switch regulatory protein 19 1 antibody
        • G0/G1 switch regulatory protein 19-1 antibody
        • G0S19 1 antibody
        • G0S19 1 protein antibody
        • Heparin binding chemotaxis protein antibody
        • L2G25B antibody
        • LD78 alpha antibody
        • LD78-alpha(4-69) antibody
        • LD78alpha antibody
        • Macrophage inflammatory protein 1 alpha antibody
        • Macrophage inflammatory protein 1-alpha antibody
        • macrophage inflammatory protein 1a antibody
        • MIP 1 alpha antibody
        • MIP 1A antibody
        • MIP-1-alpha antibody
        • MIP-1-alpha(4-69) antibody
        • MIP1 alpha antibody
        • MIP1A antibody
        • PAT 464.1 antibody
        • SCYA 3 antibody
        • SCYA3 antibody
        • SIS alpha antibody
        • SIS beta antibody
        • SIS-beta antibody
        • Small inducible cytokine A3 antibody
        • small inducible cytokine A3 (homologous to mouse Mip-1a) antibody
        • Small-inducible cytokine A3 antibody
        • Tonsillar lymphocyte LD78 alpha protein antibody
        see all

      Images

      • Western blot - Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR22529-19] (ab229900)
        Western blot - Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR22529-19] (ab229900)
        All lanes : Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR22529-19] (ab229900) at 1/1000 dilution

        Lane 1 : Untreated THP-1 (human monocytic leukemia cell line) whole cell lysate
        Lane 2 : THP-1 (treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 56 hours, followed by adding 1 µg/ml lipopolysaccharide (LPS) for a further 16 hours ) whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size: 10 kDa
        Observed band size: 12 kDa
        why is the actual band size different from the predicted?


        Exposure time: 3 minutes


        Blocking and dilution buffer: 5% NFDM/TBST.

        This blot was developed using a higher sensitivity ECL substrate.

        The molecular weight observed is consistent with what has been described in the literature (PMID: 11369791)

      • Immunoprecipitation - Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR22529-19] (ab229900)
        Immunoprecipitation - Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR22529-19] (ab229900)

        Macrophage Inflammatory Protein 1 alpha / CCL3 was immunoprecipitated from 0.35 mg of THP-1 (human monocytic leukemia cell line) (treated with Phorbol-12-myristate-13-acetate (100ng/ml) for 56h, followed by adding Lipopolysaccharide (1ug/ml) for a further 16h ) whole cell lysate with ab229900 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229900 at 1/1000 dilution.

        VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

        Lane 1: THP-1 (human monocytic leukemia cell line) (treated with Phorbol-12-myristate-13-acetate (100ng/ml) for 56h, followed by adding Lipopolysaccharide (1ug/ml) for a further 16h ) whole cell lysate 10 μg (Input).
        Lane 2: ab229900 IP in THP-1 (treated as above) whole lysate.
        Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229900 in THP-1 (treated as above) whole cell lysate.

        Blocking and dilution buffer and concentration: NFDM/TBST.
        Exposure time: 40 seconds.

      • Flow Cytometry - Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR22529-19] (ab229900)
        Flow Cytometry - Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR22529-19] (ab229900)

        Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized THP-1 (human monocytic leukemia cell line) (untreated, green) / (treated with Phorbol-12-myristate-13-acetate (100ng/ml) for 56h, followed by adding Lipopolysaccharide (1ug/ml) for a further 16h, red) cells labeling Macrophage Inflammatory Protein 1 alpha / CCL3 with ab229900 at 1/500 dilution compared with a Rabbit monoclonal IgG Isotype control details (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

      • Immunocytochemistry/ Immunofluorescence - Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR22529-19] (ab229900)
        Immunocytochemistry/ Immunofluorescence - Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR22529-19] (ab229900)

        Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 (human monocytic leukemia cell line) cells labeling Macrophage Inflammatory Protein 1 alpha / CCL3 with ab229900 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in THP-1 treated with Phorbol-12-myristate-13-acetate (100ng/ml) for 56 h, followed by adding Lipopolysaccharide (1ug/ml) for a further 16 h. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

        Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

        Treated: Cells treated with Phorbol-12-myristate-13-acetate (100ng/ml) for 56 h, followed by adding Lipopolysaccharide
        (1ug/ml) for a further 16 h.

      • Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR22529-19] (ab229900)
        Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR22529-19] (ab229900)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab229900? Please let us know so that we can cite the reference in this datasheet.

      ab229900 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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