Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody (ab9673)

Overview

  • Product name
    Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody
    See all Macrophage Inflammatory Protein 1 alpha / CCL3 primary antibodies
  • Description
    Goat polyclonal to Macrophage Inflammatory Protein 1 alpha / CCL3
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, ELISA, Neutralising, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hMIP-1-alpha (Human macrophage inflammatory protein-1 alpha)

  • Positive control
    • Recombinant human Macrophage Inflammatory Protein 1 alpha / CCL3 (ab9674) can be used as a positive control in WB.

Properties

Applications

Our Abpromise guarantee covers the use of ab9673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. To detect hMIP-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent dilution. To detect hMIP-1-alpha by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIP-1-alpha.
Neutralising Use at an assay dependent dilution.
Sandwich ELISA Use at an assay dependent dilution. Can be used as Detection antibody.

Target

  • Function
    Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C C motif chemokine 3 antibody
    • CCL 3 antibody
    • CCL3 antibody
    • CCL3_HUMAN antibody
    • Chemokine C C motif ligand 3 antibody
    • G0/G1 switch regulatory protein 19 1 antibody
    • G0/G1 switch regulatory protein 19-1 antibody
    • G0S19 1 antibody
    • G0S19 1 protein antibody
    • Heparin binding chemotaxis protein antibody
    • L2G25B antibody
    • LD78 alpha antibody
    • LD78-alpha(4-69) antibody
    • LD78alpha antibody
    • Macrophage inflammatory protein 1 alpha antibody
    • Macrophage inflammatory protein 1-alpha antibody
    • MIP 1 alpha antibody
    • MIP 1A antibody
    • MIP-1-alpha antibody
    • MIP-1-alpha(4-69) antibody
    • MIP1A antibody
    • PAT 464.1 antibody
    • SCYA 3 antibody
    • SCYA3 antibody
    • SIS alpha antibody
    • SIS beta antibody
    • SIS-beta antibody
    • Small inducible cytokine A3 antibody
    • small inducible cytokine A3 (homologous to mouse Mip-1a) antibody
    • Small-inducible cytokine A3 antibody
    • Tonsillar lymphocyte LD78 alpha protein antibody
    see all

References

This product has been referenced in:
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting Abcam.

All of the antibodies that you mentioned are guaranteed under our Abpromise to work in a Neutralizing assay, in various species:

ab89546 - Works human samples
ab9673 – Works in human samples
ab9927- Works in mouse samples
ab10381- Works in mouse and human
ab10382 -Works in mouse samples

If you are interested in larger quantities, then either just let me know which of the above antibodies you are interested in and I will pass your information onto our in-house sales team that specialize in bulk sales and they will be able to get you the best pricing possible.

If there is anything else I can help you with, please let me know.

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Answer

Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab9673 in IHC. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support but please bear in mind that this product has not been tested in IHC application and therefore, our Abpromise is not applicable. I have looked through the protocols you used and have a few questions and suggestions that might help you resolve the problem. I noticed that the primary incubation time was 24hr to 72hr, which maybe too long. Can you please try incubating overnight (not more than 18 hours) at 4oC, if you have not already done so. Increasing the dilution to 1:1000 ~ 1:5000, and increasing the washing duration will also help eliminate high background. You can try washing for 3 x 15min. Can I confirm that in your negative controls, omission of the primary antibody no longer cause high background? If so, then the problem lies with the primary antibody. It could be binding non-specifically to your sample. If the above suggestions did not prove to help you get better results, then I am afraid that this product is indeed not suitable for IHC application. Although you did not managed to obtain positive results, can I encourage you to submit an Abreview via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to other researchers. We reward each Abreview with Abpoints which can be used for discounts off future purchases and gifts. To find out more about our Abreview system, please see the following URL: https://www.abcam.com/abreviews Should you require any further information or assistance, please do not hesitate to contact me.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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