Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody (ab9927)

Overview

  • Product name
    Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody
    See all Macrophage Inflammatory Protein 1 alpha / CCL3 primary antibodies
  • Description
    Rabbit polyclonal to Macrophage Inflammatory Protein 1 alpha / CCL3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, Neutralising, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Highly pure (>98%) recombinant mMIP-1a (Mousemacrophage inflammatory protein-1 alpha)

  • Positive control
    • Recombinant mouse Macrophage Inflammatory Protein 1 alpha / CCL3 (ab9928) can be used as a positive control in WB.

Properties

Applications

Our Abpromise guarantee covers the use of ab9927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.5 µg/ml. (using 100µl/well antibody solution). Allows the detection of 0.2 - 0.4 ng/well of recombinant mMIP-1a.
Neutralising Use a concentration of 2 - 5 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of mMIP-1a (10 ng/ml).
WB Use a concentration of 0.1 - 0.2 µg/ml.

Target

  • Function
    Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C C motif chemokine 3 antibody
    • CCL 3 antibody
    • CCL3 antibody
    • CCL3_HUMAN antibody
    • Chemokine C C motif ligand 3 antibody
    • G0/G1 switch regulatory protein 19 1 antibody
    • G0/G1 switch regulatory protein 19-1 antibody
    • G0S19 1 antibody
    • G0S19 1 protein antibody
    • Heparin binding chemotaxis protein antibody
    • L2G25B antibody
    • LD78 alpha antibody
    • LD78-alpha(4-69) antibody
    • LD78alpha antibody
    • Macrophage inflammatory protein 1 alpha antibody
    • Macrophage inflammatory protein 1-alpha antibody
    • MIP 1 alpha antibody
    • MIP 1A antibody
    • MIP-1-alpha antibody
    • MIP-1-alpha(4-69) antibody
    • MIP1A antibody
    • PAT 464.1 antibody
    • SCYA 3 antibody
    • SCYA3 antibody
    • SIS alpha antibody
    • SIS beta antibody
    • SIS-beta antibody
    • Small inducible cytokine A3 antibody
    • small inducible cytokine A3 (homologous to mouse Mip-1a) antibody
    • Small-inducible cytokine A3 antibody
    • Tonsillar lymphocyte LD78 alpha protein antibody
    see all

References

ab9927 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for your reply.

For both antibodies, we guarantee them on mouse samples, but we have not necessarily tested them in in-vivo studies.

For ab9927, we recommend to start with a concentration of 2 - 5 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of mMIP-1a (10 ng/ml).

For ab10382, mouse MIP1 alpha was tested for its ability to neutralize the bioactivity of recombinant, mouse MIP1 alpha in a cell proliferation assay using hematopoietic stem cells at a concentration of 3 µg/ml.

As to how much you would need for 20 mouse, I am unable to provide a good estimate of that and would requires some optimization at your end before placing a larger order.

If there is anything else I can help you with, please let me know.

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Answer

Thank you for contacting Abcam.

All of the antibodies that you mentioned are guaranteed under our Abpromise to work in a Neutralizing assay, in various species:

ab89546 - Works human samples
ab9673 – Works in human samples
ab9927- Works in mouse samples
ab10381- Works in mouse and human
ab10382 -Works in mouse samples

If you are interested in larger quantities, then either just let me know which of the above antibodies you are interested in and I will pass your information onto our in-house sales team that specialize in bulk sales and they will be able to get you the best pricing possible.

If there is anything else I can help you with, please let me know.

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Question
Answer

DISCOUNT CODE: **********
Expiration date: *

I am very pleased to hear you would like to accept our offer and test ab25128 in IF. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

The band should be at approx 7.8 kDa. As far as a control you could use recombinant mMurine MIP1-alpha or the following tissues that should express Murine MIP1 alpha : Activated T-Cells, B-Cells, Langerhans cells, neutrophils, and macrophages all produce MIP1 alpha when stimulated with bacterial Endotoxin or cytokines.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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