Anti-Macrophage Inflammatory Protein 1 gamma antibody (ab9913)

Product name

Anti-Macrophage Inflammatory Protein 1 gamma antibody
Description

Rabbit polyclonal to Macrophage Inflammatory Protein 1 gamma
Host species

Rabbit
Tested applications

Suitable for: IHC-P, ELISA, WBmore details
Species reactivity

Reacts with: Mouse
Immunogen

Highly pure (>98%) recombinant mMIP-1g (Mouse macrophage inflammatory protein-1 gamma)
Positive control
- Recombinant mouse Macrophage Inflammatory Protein 1 gamma (ab9914) can be used as a positive control in WB.

Form

Lyophilised:Reconstitute with 200µl of sterile water.
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage buffer

PBS, pH 7.4, no preservative, sterile filtered
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Polyclonal
Isotype

unknown
Light chain type

unknown
Research areas

Our Abpromise guarantee covers the use of ab9913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P		Use at an assay dependent dilution. PubMed: 20799334
ELISA		Use at an assay dependent dilution. To detect mMIP-1g by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mMIP-1g.
WB		Use at an assay dependent dilution. To detect mMIP-1g by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-1g is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Function

Monokine with inflammatory, pyrogenic and chemokinetic properties. Circulates at high concentrations in the blood of healthy animals. Binding to a high-affinity receptor activates calcium release in neutrophils. It also inhibits colony formation of bone marrow myeloid immature progenitors.
Tissue specificity

Expressed mainly in the liver, lung, and the thymus, although some expression has been detected in a wide variety of tissues except brain.
Sequence similarities

Belongs to the intercrine beta (chemokine CC) family.
Cellular localization

Secreted.
Target information above from: UniProt accession P51670 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 20308 Mouse
- SwissProt: P51670 Mouse
- Unigene: 416125 Mouse
Alternative names
- CCF18 antibody
- Ccl9 antibody
- CCL9(31-101) antibody
- CCL9_MOUSE antibody
- Chemokine CC motif ligand 9 antibody
- Macrophage inflammatory protein 1-gamma antibody
- Macrophage inflammatory protein-related protein 2 antibody
- MIP-1-gamma antibody
- MRP-2 antibody
- SCYA10 antibody
- SCYA9 antibody
- Small inducible cytokine A9 antibody
- Small-inducible cytokine A9 antibody
see all

Cottle DL et al. Fetal inhibition of inflammation improves disease phenotypes in harlequin ichthyosis. Hum Mol Genet N/A:N/A (2014). Mouse . Read more (PubMed: 25209981) »
Simon L et al. ETV5 regulates sertoli cell chemokines involved in mouse stem/progenitor spermatogonia maintenance. Stem Cells 28:1882-92 (2010). IHC-P ; Mouse . Read more (PubMed: 20799334) »

Publishing research using ab9913? Please let us know so that we can cite the reference in this datasheet.

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