Anti-Macrophage Inflammatory Protein 1 gamma antibody (ab9913)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-Macrophage Inflammatory Protein 1 gamma antibody
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DescriptionRabbit polyclonal to Macrophage Inflammatory Protein 1 gamma
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, ELISA, WBmore details
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Species reactivityReacts with: Mouse
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Immunogen
Highly pure (>98%) recombinant mMIP-1g (Mouse macrophage inflammatory protein-1 gamma)
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Positive control
- Recombinant mouse Macrophage Inflammatory Protein 1 gamma (ab9914) can be used as a positive control in WB.
Properties
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FormLyophilised:Reconstitute with 200µl of sterile water.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPBS, pH 7.4, no preservative, sterile filtered
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Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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Isotypeunknown
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Light chain typeunknown
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab9913 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent dilution. PubMed: 20799334 | |
| ELISA | Use at an assay dependent dilution. To detect mMIP-1g by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mMIP-1g. | |
| WB | Use at an assay dependent dilution. To detect mMIP-1g by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-1g is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Target
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FunctionMonokine with inflammatory, pyrogenic and chemokinetic properties. Circulates at high concentrations in the blood of healthy animals. Binding to a high-affinity receptor activates calcium release in neutrophils. It also inhibits colony formation of bone marrow myeloid immature progenitors.
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Tissue specificityExpressed mainly in the liver, lung, and the thymus, although some expression has been detected in a wide variety of tissues except brain.
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Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
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Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 20308 Mouse
- SwissProt: P51670 Mouse
- Unigene: 416125 Mouse
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Alternative names
- CCF18 antibody
- Ccl9 antibody
- CCL9(31-101) antibody
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Datasheets and documents
References
This product has been referenced in:
- Cottle DL et al. Fetal inhibition of inflammation improves disease phenotypes in harlequin ichthyosis. Hum Mol Genet N/A:N/A (2014). Mouse . Read more (PubMed: 25209981) »
- Simon L et al. ETV5 regulates sertoli cell chemokines involved in mouse stem/progenitor spermatogonia maintenance. Stem Cells 28:1882-92 (2010). IHC-P ; Mouse . Read more (PubMed: 20799334) »
