Overview

  • Product name

    Anti-Macrophage Inflammatory Protein 3 alpha antibody
    See all Macrophage Inflammatory Protein 3 alpha primary antibodies
  • Description

    Mouse monoclonal to Macrophage Inflammatory Protein 3 alpha
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length Human Macrophage Inflammatory Protein 3 alpha produced in E.coli.

  • Positive control

    • Human spleen tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab136904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 0.4 µg/ml. Predicted molecular weight: 10 kDa. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human MIP-3a is 0.25-0.50 ng/lane, under non-reducing conditions.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA Use a concentration of 2 - 4 µg/ml.

Target

  • Function

    Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213.
  • Tissue specificity

    Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications

    C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta chemokine exodus 1 antibody
    • Beta-chemokine exodus-1 antibody
    • C C motif chemokine ligand 20 antibody
    • C-C motif chemokine 20 antibody
    • CC chemokine LARC antibody
    • Ccl20 antibody
    • CCL20(2-70) antibody
    • CCL20_HUMAN antibody
    • Chemokine (C C motif) ligand 20 antibody
    • Chemokine C-C motif chemokine 20 antibody
    • Chemokine CC motif ligand 20 antibody
    • CKb4 antibody
    • Exodus 1 antibody
    • Exodus antibody
    • LARC antibody
    • Liver and activation regulated chemokine antibody
    • Liver and activation-regulated chemokine antibody
    • Macrophage inflammatory protein 3 alpha antibody
    • MIP 3 alpha antibody
    • MIP 3A antibody
    • MIP-3-alpha antibody
    • MIP-3a antibody
    • MIP3 alpha antibody
    • MIP3A antibody
    • SCYA20 antibody
    • Small inducible cytokine A20 antibody
    • Small inducible cytokine subfamily A (Cys Cys) member 20 antibody
    • Small-inducible cytokine A20 antibody
    • ST38 antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human spleen tissue labelling Macrophage Inflammatory Protein 3 alpha with ab136904 at 10 µg/ml.

References

This product has been referenced in:

  • Tu JF  et al. Regulatory T cells, especially ICOS+ FOXP3+ regulatory T cells, are increased in the hepatocellular carcinoma microenvironment and predict reduced survival. Sci Rep 6:35056 (2016). Read more (PubMed: 27725696) »
See 1 Publication for this product

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