Overview

  • Product name

    Anti-Macrophage Inflammatory Protein 3 alpha antibody
    See all Macrophage Inflammatory Protein 3 alpha primary antibodies
  • Description

    Rabbit polyclonal to Macrophage Inflammatory Protein 3 alpha
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse Macrophage Inflammatory Protein 3 alpha aa 50 to the C-terminus conjugated to keyhole limpet haemocyanin.
    Database link: O89093

  • Positive control

    • IHC-P: FFPE Mouse spleen tissue sections. IF/ICC: RAW 246.7 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab139585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213.
  • Tissue specificity

    Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications

    C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta-chemokine exodus-1 antibody
    • C C motif chemokine ligand 20 antibody
    • C-C motif chemokine 20 antibody
    • CC chemokine LARC antibody
    • Ccl20 antibody
    • CCL20(2-70) antibody
    • CCL20_HUMAN antibody
    • Chemokine (C C motif) ligand 20 antibody
    • Chemokine CC motif ligand 20 antibody
    • CKb4 antibody
    • Exodus 1 antibody
    • Exodus antibody
    • LARC antibody
    • Liver and activation-regulated chemokine antibody
    • Macrophage inflammatory protein 3 alpha antibody
    • MIP 3 alpha antibody
    • MIP 3A antibody
    • MIP-3-alpha antibody
    • MIP-3a antibody
    • MIP3A antibody
    • SCYA20 antibody
    • Small inducible cytokine A20 antibody
    • Small inducible cytokine subfamily A (Cys Cys) member 20 antibody
    • Small-inducible cytokine A20 antibody
    • ST38 antibody
    see all

Images

  • ab139585 stained RAW 246.7 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab139585 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • IHC image of Macrophage Inflammatory Protein 3 alpha staining in mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab139585, 5µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

ab139585 has not yet been referenced specifically in any publications.

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