Overview

  • Product name
    Anti-Macrophage Inflammatory Protein 3 alpha antibody
    See all Macrophage Inflammatory Protein 3 alpha primary antibodies
  • Description
    Rabbit polyclonal to Macrophage Inflammatory Protein 3 alpha
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-FoFr, IHC-P, WB, ELISA, Neutralising, ICC/IF, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Highly pure (>98%) recombinant hMIP-3-alpha (human macrophage inflammatory protein-3 alpha)

  • Positive control
    • Recombinant human Macrophage Inflammatory Protein 3 alpha (ab9830) can be used as a positive control in WB.

Properties

Applications

Our Abpromise guarantee covers the use of ab9829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 19305396
IHC-P Use a concentration of 15 µg/ml.
WB Use at an assay dependent concentration. To detect hMIP-3-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-3-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. To detect hMIP-3-alpha by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIP-3-alpha.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-3-alpha (100 ng/ml), a concentration of 15.0 - 18.0 µg/ml of this antibody is required.
ICC/IF Use at an assay dependent concentration. PubMed: 20952674
ICC Use at an assay dependent concentration. PubMed: 24633013

Target

  • Function
    Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213.
  • Tissue specificity
    Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon.
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta-chemokine exodus-1 antibody
    • C C motif chemokine ligand 20 antibody
    • C-C motif chemokine 20 antibody
    • CC chemokine LARC antibody
    • Ccl20 antibody
    • CCL20(2-70) antibody
    • CCL20_HUMAN antibody
    • Chemokine (C C motif) ligand 20 antibody
    • Chemokine CC motif ligand 20 antibody
    • CKb4 antibody
    • Exodus 1 antibody
    • Exodus antibody
    • LARC antibody
    • Liver and activation-regulated chemokine antibody
    • Macrophage inflammatory protein 3 alpha antibody
    • MIP 3 alpha antibody
    • MIP 3A antibody
    • MIP-3-alpha antibody
    • MIP-3a antibody
    • MIP3A antibody
    • SCYA20 antibody
    • Small inducible cytokine A20 antibody
    • Small inducible cytokine subfamily A (Cys Cys) member 20 antibody
    • Small-inducible cytokine A20 antibody
    • ST38 antibody
    see all

Images

  • ab9829 (1µg/ml) staining Macrophage Inflammatory Protein 3 alpha in human tonsil (left panel) using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of vesicles.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

  • Immunohistochemical analysis of pancreatic ductal adenocarcinoma tissue, staining Macrophage Inflammatory Protein 3 alpha with ab9829.

    Antigen retrieval was performed by heat mediation in citrate buffer (pH 6.0). Tissue was blocked with 1% BSA for 10 min. The samples incubated overnight with primary antibody (1/40). Staining was detected using DAB. Sections were counterstained with hematoxylin.

References

This product has been referenced in:
  • Wang CY  et al. Effect of Liuweibuqi capsules on CD4+CD25+Foxp3+ regulatory T cells, helper T cells and lung function in patients with stable chronic obstructive pulmonary disease complicated with lung Qi deficiency. J Thorac Dis 10:2700-2711 (2018). Read more (PubMed: 29997932) »
  • Cecchinato V  et al. Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization. J Immunol 198:184-195 (2017). IHC-P . Read more (PubMed: 27895171) »
See all 13 Publications for this product

Customer reviews and Q&As

1-10 of 15 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
soution from Dako as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate
Sample
Human Tissue sections (tonsil)
Specification
tonsil
Permeabilization
No
Fixative
Formaldehyde

Dr. R Berahovich

Verified customer

Submitted Aug 06 2013

Answer

This Rabbit polyclonal is a purified antibody with a IgG concentration of 0.5MG/ML. Humans, mice, and rats have multiple IgG subclasses, whereas rabbits have only a single class of IgG. As this is a polyclonal the product is result of many different cells expressing antibodies to the target. Antibodies are produced by B lymphocytes, each expressing only one class of light chain. Once set, light chain class remains fixed for the life of the B lymphocyte. In a healthy individual, the total kappa to lambda ratio is roughly 2:1 in serum (measuring intact whole antibodies) We recommend use of the following as an isotype control: https://www.abcam.com/ab27478.html

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Answer

Gracias por contactarnos.

Para testar este anticuerpo en Western Blot usamos la proteína recombinante MIP-3 alfa diluida. Respecto al protocolo usado, se usó una concentración de anticuerpo de 0.1-0.2 ug/ml, y condiciones reductoras de la muestra.

El resto de condiciones del protocolo son las estándares de un ensayo de Western Blot. Puedes encontrar más información sobre este protocolo en https://www.abcam.com/index.html?pageconfig=resource&rid=11375.

Te recuerdo que todos nuestros productos tienen un año de garantía. En caso de no funcionar tal y como se especifica en su datasheet se reemplazará el producto o reembolsará el importe del mismo.

Espero que esta información sea de utilidad. No dudes contactarnos de nuevo para cualquier otra duda.

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Answer

Thank you for contacting us.

According to the Unitprot entry for this proteinhttp://www.uniprot.org/uniprot/O89093, CCL20 is prominently expressed in the small intestine, colon and appendix. It is also found in thymus, spleen, lymph node and lung.

This product has also been used on mouse brain in IHC-P inReboldi A et al. C-C chemokine receptor 6-regulated entry of TH-17 cells into the CNS through the choroid plexus is required for the initiation of EAE. Nat Immunol 10:514-23 (2009). IHC-Fr, IHC-P; Human, Mouse. PubMed: 19305396

We would recommend using the same tissue type as referenced in the above publication for control as data is available however you should be able to see CCL20 in any of the tissue types listed in the Uniprot entry.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Lymph Node)
Specification
Lymph Node
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No

Abcam user community

Verified customer

Submitted Jul 03 2012

Question
Answer

DISCOUNT CODE: ***
Expiration date: *********

I am very pleased to hear you would like to accept our offer and test ab9829 inrat tissue. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview forrat tissueand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Question
Answer

email bien reçu. J'ai mis en place l'envoi de l'anticorps de remplacement et je vous ai communiqué le numéro de cette commande gratuite. Avez-vous bien reçu cette confirmation de votre coté?

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Answer

Merci de nous avoir contactés.

Comme convenu j'ai mis en place l'envoi d'une unité gratuitede ab85032 en remplacement du ab9829 qui n'a pas fonctionné comme attendu en IHC-P. Le numéro de commande de remplacement gratuit de ce produit est *****. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

N'hésitez pas à nous contacter lors d'une prochaine occasion.

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Answer

as a replacement we can offer :
https://www.abcam.com/ab85032

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Answer

Thank you very much for checking with the customer.

I am very sorry to hear that the suggestions did not help to improve the results. I must conclude that the antibody vial has been faulty. I am very sorry for this inconvenience and would like to offer the customer either a credit note or a replacement antibody.

Please let me know if the customer would like to accept this. Please let me know also if he has any additional questions or concerns.

I am looking forward to your reply.

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1-10 of 15 Abreviews or Q&A

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