Overview

  • Product name
    Anti-Macrophage Inflammatory Protein 3 alpha antibody
    See all Macrophage Inflammatory Protein 3 alpha primary antibodies
  • Description
    Rabbit polyclonal to Macrophage Inflammatory Protein 3 alpha
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-FoFr, IHC-P, WB, ELISA, Neutralising, ICC/IF, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Highly pure (>98%) recombinant hMIP-3-alpha (human macrophage inflammatory protein-3 alpha)

  • Positive control
    • Recombinant human Macrophage Inflammatory Protein 3 alpha (ab9830) can be used as a positive control in WB.

Properties

Applications

Our Abpromise guarantee covers the use of ab9829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 19305396
IHC-P Use a concentration of 15 µg/ml.
WB Use at an assay dependent concentration. To detect hMIP-3-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-3-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. To detect hMIP-3-alpha by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIP-3-alpha.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-3-alpha (100 ng/ml), a concentration of 15.0 - 18.0 µg/ml of this antibody is required.
ICC/IF Use at an assay dependent concentration. PubMed: 20952674
ICC Use at an assay dependent concentration. PubMed: 24633013

Target

  • Function
    Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213.
  • Tissue specificity
    Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon.
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta-chemokine exodus-1 antibody
    • C C motif chemokine ligand 20 antibody
    • C-C motif chemokine 20 antibody
    • CC chemokine LARC antibody
    • Ccl20 antibody
    • CCL20(2-70) antibody
    • CCL20_HUMAN antibody
    • Chemokine (C C motif) ligand 20 antibody
    • Chemokine CC motif ligand 20 antibody
    • CKb4 antibody
    • Exodus 1 antibody
    • Exodus antibody
    • LARC antibody
    • Liver and activation-regulated chemokine antibody
    • Macrophage inflammatory protein 3 alpha antibody
    • MIP 3 alpha antibody
    • MIP 3A antibody
    • MIP-3-alpha antibody
    • MIP-3a antibody
    • MIP3A antibody
    • SCYA20 antibody
    • Small inducible cytokine A20 antibody
    • Small inducible cytokine subfamily A (Cys Cys) member 20 antibody
    • Small-inducible cytokine A20 antibody
    • ST38 antibody
    see all

Images

  • ab9829 (1µg/ml) staining Macrophage Inflammatory Protein 3 alpha in human tonsil (left panel) using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of vesicles.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

  • Immunohistochemical analysis of pancreatic ductal adenocarcinoma tissue, staining Macrophage Inflammatory Protein 3 alpha with ab9829.

    Antigen retrieval was performed by heat mediation in citrate buffer (pH 6.0). Tissue was blocked with 1% BSA for 10 min. The samples incubated overnight with primary antibody (1/40). Staining was detected using DAB. Sections were counterstained with hematoxylin.

References

This product has been referenced in:
  • Peled A  et al. Loss-of-function mutations in caspase recruitment domain-containing protein 14 (CARD14) are associated with a severe variant of atopic dermatitis. J Allergy Clin Immunol 143:173-181.e10 (2019). Read more (PubMed: 30248356) »
  • Zhang XF  et al. MiR-188-3p upregulation results in the inhibition of macrophage proinflammatory activities and atherosclerosis in ApoE-deficient mice. Thromb Res 171:55-61 (2018). Read more (PubMed: 30253270) »
See all 16 Publications for this product

Customer reviews and Q&As

Filter by Application

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1-3 of 3 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
soution from Dako as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate
Sample
Human Tissue sections (tonsil)
Specification
tonsil
Permeabilization
No
Fixative
Formaldehyde

Dr. R Berahovich

Verified customer

Submitted Aug 06 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Lymph Node)
Specification
Lymph Node
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No

Abcam user community

Verified customer

Submitted Jul 03 2012

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Liver)
Specification
Liver
Fixative
1:1 Acetone:Methanol
Permeabilization
No

Abcam user community

Verified customer

Submitted Feb 08 2012

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