Product nameAnti-Macrophage Inflammatory Protein 3 alpha antibody (Biotin)
See all Macrophage Inflammatory Protein 3 alpha primary antibodies
DescriptionRabbit polyclonal to Macrophage Inflammatory Protein 3 alpha (Biotin)
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Human
- WB: Recombinant human Macrophage Inflammatory Protein 3 alpha.
FormLyophilised:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.20
Constituents: PBS, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab271284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.25 - 1 µg/ml.
Using ab271284 in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human Macrophage Inflammatory Protein 3 alpha.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 10 kDa.
When used in conjunction with compatible secondary reagents, the detection limit for recombinant human Macrophage Inflammatory Protein 3 alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
FunctionChemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213.
Tissue specificityExpressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon.
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
modificationsC-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
- Information by UniProt
- Beta chemokine exodus 1 antibody
- Beta-chemokine exodus-1 antibody
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Lane 1: Marker.
Lane 2: Recombinant hMIP3 alpha at 250ng/lane.
Lane 3: Recombinant hMIP3 alpha at 125ng/lane.
Lane 4: Recombinant hMIP3 alpha at 62.5ng/lane.
Lane 5: Recombinant hMIP3 alpha at 31.25ng/lane.
Lane 6: Recombinant hMIP3 alpha at 15.625ng/lane.
Lane 7: Recombinant hMIP3 alpha at 7.8ng/lane.
Lane 8: Recombinant hMIP3 alpha at 3.9ng/lane.
Lane 9: Recombinant hMIP3 alpha at 1.95ng/lane.
Lane 10: Recombinant hMIP3 alpha at 0.975ng/lane.
Lane 11: Recombinant hMIP3 alpha at 0.4875ng/lane.
Lane 12: Recombinant hMIP3 alpha at 0.24ng/lane.
Human Macrophage Inflammatory Protein 3 alpha was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271284. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Macrophage Inflammatory Protein 3 alpha protein.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271284 has not yet been referenced specifically in any publications.