Recombinant
RabMAb

Recombinant Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22376-58] to Macrophage Inflammatory Protein 3 alpha
  • Suitable for: IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58]
    See all Macrophage Inflammatory Protein 3 alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR22376-58] to Macrophage Inflammatory Protein 3 alpha

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human Macrophage Inflammatory Protein 3 alpha aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P78556

  • Positive control

    • IHC-P: Human tonsil and Human colon tissues. ICC/IF: THP-1 cells. Flow Cyt: THP-1 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab224188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000.

 

 
ICC/IF 1/100.
Flow Cyt 1/600.

 

 
  • Application notes
    Is unsuitable for IP or WB.

    • Target

    • Function

      Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213.

    • Tissue specificity

      Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon.

    • Sequence similarities

      Belongs to the intercrine beta (chemokine CC) family.

    • Post-translational
      modifications

      C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • Beta chemokine exodus 1 antibody
        • Beta-chemokine exodus-1 antibody
        • C C motif chemokine ligand 20 antibody
        • C-C motif chemokine 20 antibody
        • CC chemokine LARC antibody
        • Ccl20 antibody
        • CCL20(2-70) antibody
        • CCL20_HUMAN antibody
        • Chemokine (C C motif) ligand 20 antibody
        • Chemokine C-C motif chemokine 20 antibody
        • Chemokine CC motif ligand 20 antibody
        • CKb4 antibody
        • Exodus 1 antibody
        • Exodus antibody
        • LARC antibody
        • Liver and activation regulated chemokine antibody
        • Liver and activation-regulated chemokine antibody
        • Macrophage inflammatory protein 3 alpha antibody
        • MIP 3 alpha antibody
        • MIP 3A antibody
        • MIP-3-alpha antibody
        • MIP-3a antibody
        • MIP3 alpha antibody
        • MIP3A antibody
        • SCYA20 antibody
        • Small inducible cytokine A20 antibody
        • Small inducible cytokine subfamily A (Cys Cys) member 20 antibody
        • Small-inducible cytokine A20 antibody
        • ST38 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)
        Immunocytochemistry/ Immunofluorescence - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)
        Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling Macrophage Inflammatory Protein 3 alpha with ab224188 at 1/100 dilution, followed by Ab224188 anti-Macrophage Inflammatory Protein 3 alpha ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing increased cytoplasmic staining in THP-1 cells treated with Phorbol-12-myristate-13-acetate (PMA, 100ng/ml) for 56 h, then together with lipopolysaccharide (1ug/ml) and Brefeldin A (300ng/ml) for another 16 h is observed. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

        Secondary antibody only control: Secondary antibody is Ab224188 anti-Macrophage Inflammatory Protein 3 alpha ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)

        Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Macrophage Inflammatory Protein 3 alpha with ab224188 at 1/2000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the epithelial like cells on the human tonsil (PMID: 15307135). Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

        Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      • Flow Cytometry - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)
        Flow Cytometry - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)

        Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized THP-1 (Human monocytic leukemia monocyte) treated with 100ng/ml PMA for 56h, then together with 1ug/ml lipopolysaccharide and 300ng/ml Brefeldin A for another 16h (Red) / Untreated control (Green) cells labelling Macrophage Inflammatory Protein 3 alpha with ab224188 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)

        Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Macrophage Inflammatory Protein 3 alpha with ab224188 at 1/2000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the follicle-associated epithelium in the human colon (PMID:14997037). Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

        Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References

      Publishing research using ab224188? Please let us know so that we can cite the reference in this datasheet.

      ab224188 has not yet been referenced specifically in any publications.

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