For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Explore the power of knock-out cell lines for your research

  1. Link

    macrophage-inflammatory-protein-3-alpha-antibody-epr22376-58-ab224188.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Innate Immunity Macrophage / Inflamm.
Share by email
RecombinantRabMAb

Recombinant Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunocytochemistry/ Immunofluorescence - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)
  • Flow Cytometry (Intracellular) - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)
  • Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22376-58] to Macrophage Inflammatory Protein 3 alpha
  • Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Knockout
Product image
Human CCL20 (Macrophage Inflammatory Protein 3 alpha) knockout HeLa cell line (ab265379)
Protein
Recombinant human Macrophage Inflammatory Protein 3 alpha (ab9830)

View more associated products

Overview

  • Product name

    Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58]
    See all Macrophage Inflammatory Protein 3 alpha primary antibodies
  • Description

    Rabbit monoclonal [EPR22376-58] to Macrophage Inflammatory Protein 3 alpha
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, ICC/IFmore details
    Unsuitable for: IP or WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil and Human colon tissues. ICC/IF: THP-1 cells. Flow Cyt (intra): THP-1 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22376-58
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Innate Immunity
    • Chemokines
    • Beta Chemokines (CC)
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cytokines and cytokine receptors ELISA kits
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] - BSA and Azide free (ab261730)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human Macrophage Inflammatory Protein 3 alpha (ab9830)
  • Related Products

    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab224188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/600.
IHC-P
1/2000.

 

 

ICC/IF
1/100.
Notes
Flow Cyt (Intra)
1/600.
IHC-P
1/2000.

 

 

ICC/IF
1/100.
Application notes
Is unsuitable for IP or WB.

Target

  • Function

    Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213.
  • Tissue specificity

    Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications

    C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P78556 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6364 Human
    • Omim: 601960 Human
    • SwissProt: P78556 Human
    • Unigene: 75498 Human
    • Alternative names

      • Beta chemokine exodus 1 antibody
      • Beta-chemokine exodus-1 antibody
      • C C motif chemokine ligand 20 antibody
      • C-C motif chemokine 20 antibody
      • CC chemokine LARC antibody
      • Ccl20 antibody
      • CCL20(2-70) antibody
      • CCL20_HUMAN antibody
      • Chemokine (C C motif) ligand 20 antibody
      • Chemokine C-C motif chemokine 20 antibody
      • Chemokine CC motif ligand 20 antibody
      • CKb4 antibody
      • Exodus 1 antibody
      • Exodus antibody
      • LARC antibody
      • Liver and activation regulated chemokine antibody
      • Liver and activation-regulated chemokine antibody
      • Macrophage inflammatory protein 3 alpha antibody
      • MIP 3 alpha antibody
      • MIP 3A antibody
      • MIP-3-alpha antibody
      • MIP-3a antibody
      • MIP3 alpha antibody
      • MIP3A antibody
      • SCYA20 antibody
      • Small inducible cytokine A20 antibody
      • Small inducible cytokine subfamily A (Cys Cys) member 20 antibody
      • Small-inducible cytokine A20 antibody
      • ST38 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)
      Immunocytochemistry/ Immunofluorescence - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)
      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling Macrophage Inflammatory Protein 3 alpha with ab224188 at 1/100 dilution, followed by Ab224188 anti-Macrophage Inflammatory Protein 3 alpha ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing increased cytoplasmic staining in THP-1 cells treated with Phorbol-12-myristate-13-acetate (PMA, 100ng/ml) for 56 h, then together with lipopolysaccharide (1ug/ml) and Brefeldin A (300ng/ml) for another 16 h is observed. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is Ab224188 anti-Macrophage Inflammatory Protein 3 alpha ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Macrophage Inflammatory Protein 3 alpha with ab224188 at 1/2000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the epithelial like cells on the human tonsil (PMID: 15307135). Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Flow Cytometry (Intracellular) - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)
      Flow Cytometry (Intracellular) - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized THP-1 (Human monocytic leukemia monocyte) treated with 100ng/ml PMA for 56h, then together with 1ug/ml lipopolysaccharide and 300ng/ml Brefeldin A for another 16h (Red) / Untreated control (Green) cells labelling Macrophage Inflammatory Protein 3 alpha with ab224188 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody. 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Macrophage Inflammatory Protein 3 alpha with ab224188 at 1/2000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the follicle-associated epithelium in the human colon (PMID:14997037). Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)
      Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab224188? Please let us know so that we can cite the reference in this datasheet.

    ab224188 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab224188.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.