Recombinant Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)
![Immunocytochemistry/ Immunofluorescence - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)](https://www.abcam.com/ps/products/224/ab224188/Images/ab224188-3-anti-macrophage-inflammatory-protein-3-alpha-antibody-epr22376-58-immunocytochemistry-thp1-human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22376-58] to Macrophage Inflammatory Protein 3 alpha
- Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58]
See all Macrophage Inflammatory Protein 3 alpha primary antibodies -
Description
Rabbit monoclonal [EPR22376-58] to Macrophage Inflammatory Protein 3 alpha -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, ICC/IFmore details
Unsuitable for: IP or WB -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human tonsil and Human colon tissues. ICC/IF: THP-1 cells. Flow Cyt (intra): THP-1 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22376-58 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/600.
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| IHC-P |
1/2000.
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| ICC/IF |
1/100.
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| Notes |
|---|
|
Flow Cyt (Intra)
1/600. |
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IHC-P
1/2000.
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ICC/IF
1/100. |
Target
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Function
Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. -
Tissue specificity
Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsC-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6364 Human
- Omim: 601960 Human
- SwissProt: P78556 Human
- Unigene: 75498 Human
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Alternative names
- Beta chemokine exodus 1 antibody
- Beta-chemokine exodus-1 antibody
- C C motif chemokine ligand 20 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling Macrophage Inflammatory Protein 3 alpha with ab224188 at 1/100 dilution, followed by Ab224188 anti-Macrophage Inflammatory Protein 3 alpha ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing increased cytoplasmic staining in THP-1 cells treated with Phorbol-12-myristate-13-acetate (PMA, 100ng/ml) for 56 h, then together with lipopolysaccharide (1ug/ml) and Brefeldin A (300ng/ml) for another 16 h is observed. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Ab224188 anti-Macrophage Inflammatory Protein 3 alpha ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)](https://www.abcam.com/ps/products/224/ab224188/Images/ab224188-1-anti-macrophage-inflammatory-protein-3-alpha-antibody-epr22376-58-immunocytochemistry-tonsil-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Macrophage Inflammatory Protein 3 alpha with ab224188 at 1/2000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the epithelial like cells on the human tonsil (PMID: 15307135). Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Flow Cytometry (Intracellular) - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)](https://www.abcam.com/ps/products/224/ab224188/Images/ab224188-4-anti-macrophage-inflammatory-protein-3-alpha-antibody-epr22376-58-flowcytometry-thp1-human.jpg)
Flow Cytometry (Intracellular) - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized THP-1 (Human monocytic leukemia monocyte) treated with 100ng/ml PMA for 56h, then together with 1ug/ml lipopolysaccharide and 300ng/ml Brefeldin A for another 16h (Red) / Untreated control (Green) cells labelling Macrophage Inflammatory Protein 3 alpha with ab224188 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)](https://www.abcam.com/ps/products/224/ab224188/Images/ab224188-2-anti-macrophage-inflammatory-protein-3-alpha-antibody-epr22376-58-immunocytochemistry-colon-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Macrophage Inflammatory Protein 3 alpha with ab224188 at 1/2000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the follicle-associated epithelium in the human colon (PMID:14997037). Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)](https://www.abcam.com/ps/products/224/ab224188/Images/ab224188-5-benefits-of-recombinant-antibodies.png)
Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab224188 has not yet been referenced specifically in any publications.
