Anti-CCL18 antibody (ab9849)
Key features and details
- Rabbit polyclonal to CCL18
- Suitable for: ELISA, Neutralising, Sandwich ELISA, WB
- Reacts with: Human
- Isotype: unknown
Overview
-
Product name
Anti-CCL18 antibody
See all CCL18 primary antibodies -
Description
Rabbit polyclonal to CCL18 -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, Neutralising, Sandwich ELISA, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to CCL18.
-
Positive control
- Recombinant human CCL18 protein (ab9850) can be used as a positive control in WB.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Lyophilized:Reconstitute with 200µl of sterile water. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
No preservative, sterile filtered -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
unknown -
Light chain type
unknown -
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9849 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
|
Neutralising |
Use at an assay dependent concentration.
|
|
Sandwich ELISA |
Use at an assay dependent concentration.
To detect CCL18 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with an appropriate detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant CCL18. . |
|
WB |
Use at an assay dependent concentration.
|
Notes |
---|
ELISA
Use at an assay dependent concentration. |
Neutralising
Use at an assay dependent concentration. |
Sandwich ELISA
Use at an assay dependent concentration. To detect CCL18 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with an appropriate detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant CCL18. . |
WB
Use at an assay dependent concentration. |
Target
-
Function
Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses. -
Tissue specificity
Expressed at high levels in lung, lymph nodes, placenta, bone marrow, dendritic cells present in germinal centers and T-cell areas of secondary lymphoid organs and macrophages derived from peripheral blood monocytes. Not expressed by peripheral blood monocytes and a monocyte-to-macrophage differentiation is a prerequisite for expression. Expressed in synovial fluids from patients with rheumatoid and septic arthritis and in ovarian carcinoma ascitic fluid. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 6362 Human
- Omim: 603757 Human
- SwissProt: P55774 Human
- Unigene: 143961 Human
-
Alternative names
- Alternative macrophage activation associated CC chemokine 1 antibody
- Alternative macrophage activation-associated CC chemokine 1 antibody
- AMAC-1 antibody
see all
Datasheets and documents
-
Datasheet download
References (4)
ab9849 has been referenced in 4 publications.
- Su S et al. Blocking the recruitment of naive CD4(+) T cells reverses immunosuppression in breast cancer. Cell Res 27:461-482 (2017). Blocking ; Human . PubMed: 28290464
- Huang D et al. Epstein-Barr Virus-Induced VEGF and GM-CSF Drive Nasopharyngeal Carcinoma Metastasis via Recruitment and Activation of Macrophages. Cancer Res 77:3591-3604 (2017). PubMed: 28484077
- Nie Y et al. Tumor-Associated Macrophages Promote Malignant Progression of Breast Phyllodes Tumors by Inducing Myofibroblast Differentiation. Cancer Res 77:3605-3618 (2017). PubMed: 28512246
- Su S et al. A positive feedback loop between mesenchymal-like cancer cells and macrophages is essential to breast cancer metastasis. Cancer Cell 25:605-20 (2014). PubMed: 24823638