Overview

  • Product name
    Anti-Macrophage Scavenger Receptor I antibody
    See all Macrophage Scavenger Receptor I primary antibodies
  • Description
    Rabbit polyclonal to Macrophage Scavenger Receptor I
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Cow, Cat, Human, Monkey
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 300-400 of Human Macrophage Scavenger Receptor I (UniProt P21757).

  • Positive control
    • Hela cells; Human liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab123946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ICC/IF 1/50 - 1/200.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Membrane glycoproteins implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis. Two types of receptor subunits exist. These receptors mediate the endocytosis of a diverse group of macromolecules, including modified low density lipoproteins (LDL). Isoform III does not internalize actetylated LDL.
  • Tissue specificity
    Isoform I, isoform II and isoform III are expressed in monocyte-derived macrophages.
  • Sequence similarities
    Contains 1 collagen-like domain.
    Contains 1 SRCR domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD204 antibody
    • CD204 antigen antibody
    • CD24 antibody
    • Macrophage acetylated LDL receptor I and II antibody
    • Macrophage scavenger receptor 1 antibody
    • Macrophage scavenger receptor type III antibody
    • Macrophage scavenger receptor types I and II antibody
    • Msr 1 antibody
    • MSR1 antibody
    • MSRE_HUMAN antibody
    • phSR1 antibody
    • phSR2 antibody
    • SCARA 1 antibody
    • SCARA1 antibody
    • Scavenger receptor class A member 1 antibody
    • Scavenger receptor class A, member 1 antibody
    • Scavenger receptor type A antibody
    • Scvr antibody
    • SR A antibody
    • SRA antibody
    see all

Images

  • Anti-Macrophage Scavenger Receptor I antibody (ab123946) at 0.5 µg/ml + Human liver lysate

    Predicted band size: 50 kDa
    Observed band size: 50 kDa

  • ab123946, at a 1/50 dilution, staining Macrophage Scavenger Receptor I (green) in Hela cells by Immunofluorescence. Nuclei (blue) are counterstained using Hoechst 33258.

References

This product has been referenced in:
  • Chang TT  et al. Fucosyl-Agalactosyl IgG1 Induces Cholangiocarcinoma Metastasis and Early Recurrence by Activating Tumor-Associated Macrophage. Cancers (Basel) 10:N/A (2018). Read more (PubMed: 30469416) »
  • Pasterk L  et al. Oxidized plasma albumin promotes platelet-endothelial crosstalk and endothelial tissue factor expression. Sci Rep 6:22104 (2016). Read more (PubMed: 26905525) »
See all 3 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Spleen)
Antigen retrieval step
Enzymatic
Permeabilization
No
Specification
Spleen
Blocking step
2.5% Normal Horse Serum - VectorLabs ImmPRESS™ Excel Amplified HRP Polymer Staining Kit (Anti-Mouse Ig) as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: RT°C
Fixative
10% Neutral Buffered Formalin

Dr. Basel Assaf

Verified customer

Submitted Jun 03 2015

Answer

Thank you for kindly confirming these details. I am sorry this vial of antibody has not worked for this customer.

As requested, we are pleased to arrange a free of charge replacement. I can confirm that 1 vial of ab17147 Anti-CD8 antibody [144B]and ab123946 Anti-Macrophage Scavenger Receptor I antibodyhave been added to your Abcam order reference 1111111. It wasn't possible to send this replacements out with your Friday order, as this will be packed before we Scientific Supporters working. I am sorry about this.

I would like to reassure you that this free of charge replacement vial is also covered by our Abpromise guarantee. Should the customer still be experiencing difficulties with the new vial, or if you have any further questions, please do not hesitate to let me know.

Thank you for your help and cooperation with this case. Please do not hesitate to contact me if you need anything further.

Read More

Question

A user have a problem about "Macrophage Scavenger Receptor I antibody (ab123946) and CD8 antibody (ab87951)" IHC staining. You can see detailed complaint, protocols on the attached files and staining photos on the links.
The user would like free of replacement of the antibodies.

I look forward your reply.


I've linked 6 files to this email:


https://www.yousendit.com/download/TEhYRE9pd0lreEFkVU1UQw(5.5 MB)https://www.yousendit.comhttps://www.yousendit.com/download/TEhYRE9pd0lreEFkVU1UQw

https://www.yousendit.com/download/TEhYRE9pd0lubVdwSHNUQw(5.5 MB)https://www.yousendit.comhttps://www.yousendit.com/download/TEhYRE9pd0lubVdwSHNUQw

https://www.yousendit.com/download/TEhYRE9pd0lveE4zZU1UQw(5.5 MB)https://www.yousendit.comhttps://www.yousendit.com/download/TEhYRE9pd0lveE4zZU1UQw

https://www.yousendit.com/download/TEhYRE9pd0k1R081eDhUQw(5.5 MB)https://www.yousendit.comhttps://www.yousendit.com/download/TEhYRE9pd0k1R081eDhUQw

https://www.yousendit.com/download/TEhYRE9pd0kwMEd4djlVag(5.5 MB)https://www.yousendit.comhttps://www.yousendit.com/download/TEhYRE9pd0kwMEd4djlVag

https://www.yousendit.com/download/TEhYRE9uQVNFc0pnRXNUQw(5.5 MB)https://www.yousendit.comhttps://www.yousendit.com/download/TEhYRE9uQVNFc0pnRXNUQw


http://www.getthunderbird.com makes it easy to share large files over email.
Antibody information:

Antibody Name: CD8
Antibody code number: ab87951
Antibody lot number: GR76570-1

How is the product stored: aliquoted and stored at -20°C


Complaint:
It was non-spesific staining and background staining.
When we performed IHC on the instrument; there wasn’t a staining.
(We tested the antibody on DAKO IHC instrument and also manuel staining)


Positive control tissue:
Human Tonsil tissue, FFPE

Fixative and Fixation time: NBF- 24 hours





IHC Detection Kit brand, name, code number: Dako Envision Flex k8000


DETAILED IHC PROTOCOL:


Pretreatment (citrate? EDTA? Or another?):

EDTA ph 9; 97C; 20 minutes in Dako PTlink instrument
Citrate pH 6; 97C 20 minutes in a Dako PTlink instrument
EDTA pH 9; 10 minutes in a microwave oven
Citrate pH 6; 5 minutes in a microwave oven
None antigen retrieval


Protein blocking incubation time:
Peroxidase block- 5 minutes and 15 minutes are tested
Protein block 10 and 15 minutes



Primary Antibody dilution and incubation time and temparature:
(Primer antikoru ne oranda dilüe edip kullandınız; inkübasyon süresiyle birlikte yazınız. İnkübasyon kaç derece yapıldı?)

1/50, 1/100 and 1/200
45 minutes, 60 minutes at RT and overnight at +4C are tested
Secondary antibody dilution and incubation time:

Secondary antibody is HRP conjugted and RTU (in the DAKO kit)
20 minutes and 30 minutes was tested.


Chromogen (DAB? AEC? Or another?) DAB Substrate-chromogen solution 10 min.



Further Comments

We contacted with KIMERA to have a technical support. A staff from KIMERA technical department came to our lab and tested the antibody by manuelly and by the instrument. She used EXPOSE kit (ab80436) and Dako Envision Flex k8000 seperately.

Unfourtunately results was same again. We think the antibody isn’t work and we would like a free of charge replacement based on ABCAM promise.

You can see the staining results in the attached files.

P.S.: We used Foxp3-ab20034 and cd68-ab955 antibodies also and they worked.

Read More
Answer

Thank you for your message and for providing this further information.

I am sorry to hear the resultsare not satiesfactoryon this occasion. I appreciate the time you and your customerhave spent on these experiments, andit is no problem to send out a free of charge replacement (providing the product has been purchased in the last 180 days). In order to arrange this, I would appreciate if you could confirm your order number and date of purchase? In addition, would the customer prefer the same antibody as a replacement or an alternative one?

Thank you for your continued cooperation. I look forward to hearing from you with details of how you would like to proceed.

Read More

Answer

Thank you for your enquiry and interest in our products.

The best positive control would be tissue sections from human liver. Bone marrow, spleen, breast tissues may be also suitable but we have not tested these tissue sections with ab123946.

Here is an interesting and useful website to visit for further information about the expression level/pattern of Macrophage Scavenger Receptor I:

http://www.proteinatlas.org/ENSG00000038945

We have a few tissue slides which may be of interest to you and your customer.

ab4348: Liver (human) normal tissue slides

ab4670: Bone tumor (human): chondroma tissue slides

ab4675: Bone tumor (human): giant cell tumor (grade I - II)

ab4677: Bone tumor (human): synovial sarcoma tissue slides

ab4372: Spleen (human) normal tissue slides

If you need any further assistance, please do not hesitate to contact me.

Read More

Answer

Thank you for your call today and for your patience while I have been in touch with the lab.

Apparently there have been mixed results with this antibody in mouse samples, and we aren't sure whether it will react with Bl/6 mice. Based on this data, I have removed mouse as a tested species. We do have some other antibodies that are predicted to react with mouse based on homology (such as ab79940) but as this is not tested we can not guarantee it. If you would like to try any of these antibodies on mouse samples, we do have a testing discount program that you might be interested in, and I'd be happy to send more details about this if you would like.

I am sorry that I don't have conclusive information about the mousereactivity of this antibody, but please let me know if you have any further questions or if there is anything else that we might be able to do for you.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up